磁性纳米金属对盐单胞菌生长特性及胞内Ectoine的积聚影响  

Effects of Magnetic Nano-metals on Growth Characteristics and Intracellular Ectoine Accumulation of Halomonas sp.XH26

作　　者：汪明香 郭敏 高翔 李永臻 韩睿[2] 朱德锐 沈国平 WANG Ming-xiang;GUO Min;GAO Xiang;LI Yong-zhen;HAN Rui;ZHU De-rui;SHEN Guo-ping(Research Center of Basic Medical Science,Medical College,Qinghai University,Xining 810016,China;Qinghai Key Laboratory of Vegetable Genetics and Physiology,Academy of Agriculture and Forestry Sciences,Qinghai University,Xining 810016,China)

机构地区：[1]青海大学医学院基础医学研究中心,西宁810016 [2]青海大学农林科学院青海省蔬菜遗传与生理重点实验室,西宁810016

出　　处：《中国生物工程杂志》2023年第5期45-54,共10页China Biotechnology

基　　金：国家自然科学基金(32260019);青海省基础应用研究计划(2022-ZJ-771)资助项目。

摘　　要：为了提高野生型坎帕尼亚盐单胞菌株(Halomonas campaniensis sp.XH26)胞内Ectoine的积聚量,选用磁性纳米金属颗粒(Fe_(3)O_(4)NPs),采用单因素实验、Plackett-Burman设计和响应面法分析菌株胞内的Ectoine积聚量和菌株生长量,并探讨Fe_(3)O_(4)NPs应用于盐单胞菌发酵的可行性。单因素分析表明Fe_(3)O_(4)NPs能促进菌株生长和促进菌株Ectoine的积聚,菌株生长对数期是最佳的Fe_(3)O_(4)NPs添加时期。Plackett-Burman及响应面分析表明:优化条件下(0.05 g/L Fe_(3)O_(4)NPs、1.53 mol/LNaCl和0.03 mol/LL-谷氨酸钠),摇瓶发酵Ectoine的积聚量可达640.28 mg/L,与野生菌株(391.35 mg/L)相比提高了63.61%。透射电镜显示Fe_(3)O_(4)NPs大量聚集于细菌细胞膜表面,可能通过表面吸附发挥催化作用。综上所述,Fe_(3)O_(4)NPs能有效促进菌株胞内Ectoine的积聚,Plackett-Burman与响应面法联用能较好的优化菌株发酵条件,为后续将磁性纳米金属应用于Ectoine的工业发酵生产,提供新的技术思路和参考依据。Objective:Ectoine,a compatible solute,is widely distributed in halophilic bacteria to resist extreme environmental conditions.The aim of this study is to increase the accumulation of ectoine in wild-type Halomonas campaniensis sp.XH26.Methods:Magnetic nanoparticles(Fe_(3)O_(4)NPs)were used,and single factor analysis,Plackett-Burman design and the response surface method were performed to evaluate the intracellular accumulation of ectoine and bacterial growth.The feasibility of nano-metal application in Halomonas fermentation was further discussed.Results:According to single factor analysis,Fe_(3)O_(4)NPs can promote the strain growth and accumulation of ectoine.The optimal period for adding Fe_(3)O_(4)NPs was the logarithmic growth stage of the strain.Plackett-burman and the response surface results revealed that ectoine accumulation of the strain reached 640.28 mg/L in shaker fermentation,under the optimized conditions(Fe_(3)O_(4)NPs:0.05 g/L;NaCl:1.53 mol/L;MSG:0.03 mol/L),which was 63.61%higher than that of the wild strain(391.35 mg/L).Transmission electron microscopy indicated that Fe_(3)O_(4)NPs accumulated on the surface of bacterial cell membrane,which may play a catalytic role through surface adsorption.Conclusions:In summary,Fe_(3)O_(4)NPs can effectively promote the accumulation of ectoine,and the combination of Plackett-Burman and the response surface method can better optimize the fermentation conditions of the strain.This study provides a new technical idea and reference for the subsequent application of magnetic nano-metal particles in the industrial production of ectoine by fermentation.

关 键 词：盐单胞菌 四氢嘧啶 Fe_(3)O_(4)NPs 高效液相色谱法 响应面法 

分 类 号：Q819[生物学—生物工程]