两种包被氨基酸过瘤胃保护效果和稳定性检验  

Evaluation of rumen protection effect and stability of two rumen-protected amino acids

作　　者：宋玲玲 袁瑞彤 张璐[1] 刘海英[1] 韩迪 豆兴堂 王春艳 SONG Ling-ling;YUAN Rui-tong;ZHANG Lu;LIU Hai-ying;HAN Di;DOU Xing-tang;WANG Chun-yan

机构地区：[1]沈阳农业大学动物科学与医学学院,辽宁沈阳110866 [2]庄河市职业教育中心,辽宁大连116400 [3]辽宁省现代农业生产基地建设工程中心,辽宁辽阳111000

出　　处：《饲料研究》2023年第10期1-4,共4页Feed Research

基　　金：国家自然科学基金(项目编号:32272901);辽宁省自然科学基金(项目编号:2022011559-JH3/107、2020-MS-202);国家绒毛用羊产业体系(项目编号:CARS-39-10、CARS-39-27);辽宁省“揭榜挂帅”科技攻关项目(项目编号:2021JH1/10400033);辽宁省科技特派团(项目编号:2022JH5/10400049)。

摘　　要：试验旨在研究半胱氨酸和蛋氨酸包被产品的唾液、瘤胃和小肠降解率,检验过瘤胃半胱氨酸(RPCys)和蛋氨酸(RPMet)包被稳定性。采用人工唾液法、半体内尼龙袋法、体外模拟小肠消化法测定RPCys和RPMet降解率,综合评定RPCys与RPMet的过瘤胃保护效果。结果表明,RPCys与RPMet在人工唾液中2 h降解率分别为3.24%和9.32%,4 h降解率分别为4.10%和11.84%;在瘤胃中的有效降解率分别为8.97%和32.00%;在体外模拟小肠环境中2 h后降解率分别高于87.38%和81.12%。研究表明,RPCys和RPMet包被效果良好,在人工唾液和瘤胃中较稳定且有较高的小肠释放率;与RPMet相比,RPCys的过瘤胃保护效果较好。The experiment was conducted to study the degradation rate of RPCys and RPMet in saliva,rumen and small intestine,examine the stability of RPCys and RPMet coating.The degradation rates of RPCys and RPMet were determined by artificial saliva method,semi-internal nylon bag method and in vitro simulated intestinal digestion method,and the protective effect of RPCys and RPMet over rumen was evaluated comprehensively.The results showed that the degradation rates of RPCys and RPMet in artificial saliva were 3.24%,9.32%in 2 h and 4.10%,11.84%in 4 h,respectively.The effective degradation rates in rumen were 8.97%and 32.00%,respectively.The degradation rate was higher than 87.38%and 81.12%after 2 h in the simulated small intestine environment in vitro,respectively.The experiment indicates that RPCys and RPMet are well coated,stable in artificial saliva and rumen,which have high small intestinal release rate.Compared with RPMet,RPCys has better rumen bypass protection effect.

关 键 词：辽宁绒山羊 过瘤胃半胱氨酸 过瘤胃蛋氨酸 唾液稳定性 瘤胃稳定性 小肠降解率 

分 类 号：S827[农业科学—畜牧学]