Effects of the vegetative propagation method on juvenility in Robinia pseudoacacia L.  

作　　者：Jie Liu Zijie Zhang Yapeng Li Juan Han Huayu Si Yueqi Mi Shaoming Wang Xiaoning Wei Hao Yang Yuhan Sun Yun Li 

机构地区：[1]Beijing Advanced Innovation Center for Tree Breeding by Molecular Design,Engineering Technology Research Center of Black Locust of National Forestry and Grassland Administration,National Engineering Laboratory for Tree Breeding,College of Biological Sciences and Technology,Beijing Forestry University,Beijing,People's Republic of China [2]State-Owned Quanbaoshan Forestry Station in Luoning County of He’nan Province,Luoyang,People's Republic of China [3]State-Owned Lvcun Forestry Farm in Luoning County of He’nan Province,Luoyang,People’s Republic of China [4]Xiaoxian Forestry Development Center of An’hui Province,Suzhou,People's Republic of China

出　　处：《Forestry Research》2022年第1期167-176,共10页林学研究（英文）

基　　金：the National Science Foundation of China(31971675 and 32171769);the National Key R&D Program of China(2017YFD0600503)。

摘　　要：Vegetative propagation is an important method of reproduction and rejuvenation in forestry.The growth and development of asexually propagated trees are influenced by the age and position of the propagule on the plant,effects referred to as cyclophysis and topophysis,respectively.Due to the long lifespans and large body sizes of woody trees,the selection of propagules is critically important.Here,we used three vegetative propagation methods(shoot cutting,root sprouting,and root cutting)to study the effect of different regeneration methods on juvenility of the resulting black locust plants,with seed-derived seedlings used as a control.Most characteristics of plantlets generated by rootsprouting were similar to those of seed-derived seedlings,including leaf traits and leaf anatomical structure.However,there were significant differences between the plantlets derived from shoot-cuttings and seedlings from seeds.Furthermore,the data showed that some of these agerelated small RNAs and genes differed in expression among propagation methods and between plantlets/seedlings and mature trees.These agerelated small RNAs,genes,and transcription factors may be used as molecular markers of juvenility and phase transitions in black locust.Our results provide useful information for the optimal propagation of woody trees and for further research into the mechanisms of root regeneration.

关 键 词：STRUCTURE PROPAGATION SIZES 

分 类 号：S792[农业科学—林木遗传育种]