基于聚硫堇修饰金电极构建无标记电流型免疫传感器检测冈田酸  

A tabel-free amperometric immunosensor for okadaic acid detection based on polythionine-modified gold electrodes

作　　者：梁晨希 曹立新 LIANG Chenxi;CAO Lixin(Department of Energy Chemistry and Materials Engineering,Shanxi Energy College,Jinzhong 030600,China;School of Marine Scienceand Technology,Harbin Institute of Technology(Weihai),Weihai 264209,China)

机构地区：[1]山西能源学院能源化学与材料工程系,山西晋中030600 [2]哈尔滨工业大学(威海)海洋科学与技术学院,山东威海264209

出　　处：《中国高新科技》2023年第9期132-133,共2页

基　　金：国家自然科学基金资助项目(21273056);山西省高等学校教学改革创新项目(J20221263)。

摘　　要：在金电极表面利用循环伏安法电聚合修饰聚硫堇(PTh),由于PTh表面具有大量的-NH_(2)官能团,可通过与戊二醛发生席夫碱反应固定抗体,基于抗原-抗体特异性免疫反应的原理,构建一种无标记电流型免疫传感器,实现对冈田酸(OA)的定量检测。用该免疫传感器以方波伏安法(SWV)对OA浓度进行检测,在0.1~100μg/L范围内,免疫反应前后峰值电流差值△i_(p)与OA浓度对数值lgC_(OA)具有良好的线性关系,线性回归方程为△i_(p)=4.1915+2.1679lgC_(OA),线性相关系数R2=0.9578,检测限为18.1 ng/L(S/N=3)。该传感器检测限低、线性工作区间宽、操作简便。Polythionine(PTh)was modified on the surface of gold electrode by cyclic voltammetry.And the antibodies were immobilized by the Schiff base reaction with glutaraldehyde due to the large number of-NH_(2) functional groups on the surface of PTh.Finally,a label-free amperometric immunosensor was constructed for the quantitative detection of okadaic acid(OA)based on the principle of antigen-antibody specific immune response.The immunosensor was used to detect the OA concentration by square wave voltammetry(SWV).In the range of 0.1~100μg/L,the peak current △i_(p),and OA concentration difference before and after the immune reaction had a linear relationship to lgC_(OA).The linear regression equation is △i_(p),=4.1915+2.1679lgC_(OA) with a linear correlation coeficient R2=0.9578.The detection limit is 18.1 ng/L(S/N=3).The sensor has low detection limit,wide linear working range and simple operation.

关 键 词：冈田酸 无标记电流型免疫传感器 聚硫堇修饰电极 

分 类 号：O657[理学—分析化学]