Modeling human pregastrulation development by 3D culture of blastoids generated from primed-to-naive transitioning intermediates  

作　　者：Zhifen Tu Yan Bi Xuehao Zhu Wenqiang Liu indian Hu Li Wu Tengyan Mao Jianfeng Zhou Hanwei Wang Hong Wang Shaorong Gao Yixuan Wang 

机构地区：[1]Translational Medical Center for Stem Cell Therapy&Institute for Regenerative Medicine,Shanghai East Hospital,School of Life Sciences and Technology,Tongji University,Shanghai200120,China [2]Shanghai Key Laboratory of Matermal Fetal Medicine,Clinical and Translational Research Center of Shanghai First Matemity and Infant Hospital,School of Life Sciences and Technology,TongjiUniversity,Shanghai 200092,China [3]Frontier Science Center for Stem Cell Research,Tongji University,Shanghai 200092,China

出　　处：《Protein & Cell》2023年第5期337-349,共13页蛋白质与细胞（英文版）

基　　金：the Ministry of Science and Technology of China(Nos.2021YFA1102000,2021YFC2700300,2019YFA0110000,and 2018YFA0108900);the National Natural Science Foundation of China(NSFC)(Nos.31721003,32022024,32270850,32100633,31871486,32070652,and 81630035);the Science and Technology Commission of Shanghai Municipality(Nos.19JC1415300 and 21JC1405500).

摘　　要：Human pluripotent stem cells provide an inexhaustible model to study human embryogenesis in vitro.Recent studies have provided diverse models to generate human blastoids by self-organization of different pluripotent stem cells or somatic reprogramming intermediates.However,whether blastoids can be generated from other cell types or whether they can recapitulate postimplantation development in vitro is unknown.Here,we develop a strategy to generate human blastoids from heterogeneous intermediates with epiblast,trophectoderm,and primitive endoderm signatures of the primed-to-naïve conversion process,which resemble natural blastocysts in morphological architecture,composition of cell lineages,transcriptome,and lineage differentiation potential.In addition,these blastoids reflect many features of human peri-implantation and pregastrulation development when further cultured in an in vitro 3D culture system.In summary,our study provides an alternative strategy to generate human blastoids and offers insights into human early embryogenesis by modeling peri-and postimplantation development in vitro.

关 键 词：blastoids primed-to-naive conversion transitioning intermediates pregastrulation modeling 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]