森林火灾后云南高原森林真菌群落响应的物种特异性  

Responses of fungal community to forest fire are speciesspecific in Yunnan Plateau,southwest China

作　　者：Ling-Ling Wang Qing-Qiu Zhou Wen-Hua Su Yun-Jian Xu Qin-Dong Qian Xiong Yang De-Yun Chen Zhe Chen Jian-Ping Wu 

机构地区：[1]Yunnan Key Laboratory of Plant Reproductive Adaptation and Evolutionary Ecology/Institute of Biodiversity,Yunnan University,Kunming 650500,China [2]Key Laboratory of Soil Ecology and Health in Universities of Yunnan Province,School of Ecology and Environmental Sciences,Yunnan University,Kunming 650500,China

出　　处：《Journal of Plant Ecology》2023年第1期37-49,共13页植物生态学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(no.31971497);the Xingdian Scholar Fund of Yunnan and by Double Top University Fund of Yunnan University。

摘　　要：森林地下部分包含了丰富的真菌多样性。森林火灾产生的高温和对地上植被的破坏,会改变真菌的群落组成并影响后期森林的恢复。云南是我国重要的林区,也是森林火灾的频发区。本文旨在研究以云南松(Pinus yunnanensis)和柠檬桉(Eucalyptus citriodora)为主要树种的混交林在林冠火发生后地下真菌的响应。实验采集了两种树种下的矿质土、根际土和细根3个位点的样品,通过高通量测序手段,分析了真菌的多样性、群落组成、网络结构和生态营养型等信息。结果发现,3个采样点之间的群落组成具有显著性差异,矿质土壤中的alpha多样性高于根际土壤和细根中的alpha多样性。对于树种而言,柠檬桉的真菌网络复杂程度高于云南松。两种树种的前10个优势属以腐生真菌为主,其中云南松的毛霉菌门相对丰度高于柠檬桉。在生物标志物的比较中,云南松的根和根际土壤中均以外生菌根真菌为主,而柠檬桉的根和根际土壤则表现为以腐生真菌和共生真菌为主。我们的研究结果表明森林火灾后云南高原典型森林中真菌群落的响应具有物种特异性。Fire frequently causes strong disturbance in forests.Belowground fungi are sensitive to forest fire disturbance and can affect vegetation restoration.Our objective was to investigate the responses of belowground fungi under two tree species to forest fire in a mixed forest in Yunnan Plateau.Samples from three‘sample sites’(roots,rhizosphere soil and bulk soil)were collected from two plant species,Pinus yunnanensis and Eucalyptus citriodora,and were analyzed for fungal diversity and community composition.Fungi were identified using high-throughput sequencing.Shannon index and principal coordinate analysis were used for diversity and network analysis.LEfSe and FUNGuild were used to analyze the community composition,trophic mode and guild.Alpha diversity in bulk soil was higher than that in rhizosphere soil and roots,while the community composition was significantly different among the three sample sites.Fungal network complexity of E.citriodora was higher than that of P.yunnanensis.The relative abundance of Mucoromycota in the three sample sites was higher for P.yunnanensis than E.citriodora.Most of the top 10 dominant genera of the two plant species were saprotrophic fungi.In the comparison of biomarker,ectomycorrhizal fungi dominated in the roots and rhizosphere soil of P.yunnanensis,while both saprotrophic fungi and symbiotic fungi were in the roots and rhizosphere soil of E.citriodora.Our findings suggest that,following a forest fire,responses of fungal community are species-specific in Yunnan Plateau.

关 键 词：真菌 林火 根际土 细根 云南松(Pinus yunnanensis) 柠檬桉(Eucalyptus citriodora) 云南高原 

分 类 号：S71[农业科学—林学]