广东地区致雏鹅痛风鹅星状病毒的鉴定及遗传演化分析  

Identification and evolutionary analysis of goose astrovirus that causing gout in gosling in Guangdong province

作　　者：侯展鹏 梁宇 王海悦 林佩仪 金少冰 刘文俊 曹楠 李婉雁 李冰心 许丹宁 田允波 黄运茂 付新亮 HOU Zhan-peng;LIANG Yu;WANG Hai-yue;LIN Pei-yi;JIN Shao-bing;LIU Wen-jun;CAO Nan;LI Wan-yan;LI Bing-xin;XU Dan-ning;TIAN Yun-bo;HUANG Yun-mao;FU Xin-liang(College of Animal Science and Technology,Zhongkai University of Agricultural and Engineering,Guangzhou 510225,China;Guangdong province key laboratory of waterfowl healthy breeding,Guangzhou 510225,China)

机构地区：[1]仲恺农业工程学院动物科技学院,广东广州510225 [2]广东省水禽健康养殖重点实验室,广东广州510225

出　　处：《中国预防兽医学报》2023年第3期310-314,318,共6页Chinese Journal of Preventive Veterinary Medicine

基　　金：广东省基础与应用基础研究基金区域联合基金-青年基金项目(2021A1515111167);广东省现代农业水禽产业技术体系创新团队(2022KJ137);广州市科技计划基础与应用基础研究项目(202102021235)。

摘　　要：为了解广东地区鹅星状病毒(GoAstV)的流行和变异情况,本研究于2019年~2021年从广东清远、广州、江门和汕尾4个地区采集83份疑似患痛风雏鹅的肝脏和肾脏病料样品。制备的组织病理切片结果显示,痛风雏鹅肝脏和肾脏组织出现大量的炎性细胞浸润和细胞坏死,经RT-PCR检测鉴定到其中72份样品为Go AstV阳性。从4个地区各选1份阳性样品对鉴定到的Go Ast V进行全基因组的PCR扩增,结果显示获得4株长度均为7033 bp的Go AstV全基因组序列。采用Meg Align分析的核苷酸同源性结果显示,4株Go Ast V全基因组序列的同源性为98.4%~99.4%,与报道的Go AstV-2参考株全基因组序列的同源性为97.1%~99.7%,而与Go AstV-1全基因组序列的同源性仅为57.3%~57.7%。与其他禽星状病毒相比,Go AstV与火鸡星状病毒2型(TAstV-2)的同源性最高,其全基因组序列同源性约为65.0%~65.3%。Cap蛋白的氨基酸序列分子特征分析结果显示,4个鉴定株Cap蛋白的氨基酸序列均发生了Y^(36)H突变,其中Go Ast V/Guangdong/NH2/2019和Go Ast V/Guangdong/GZ-HOD/2021 Cap蛋白氨基酸序列还发生了E^(456)D等突变。采用MEGA7.0软件构建Go Ast V全基因组和Cap基因的遗传进化树,结果显示Go AstV可划分为Go AstV-1和Go AstV-2两个分支,本研究鉴定到的4株Go Ast V均属于Go AstV-2分支。本研究首次初步揭示了广东地区鹅群Go AstV的流行特点和遗传变异情况,为Go AstV感染的防控和疫苗株的筛选提供参考。In order to understand the prevalence and variation of GoAstV in geese in Guangdong,83 liver and kidney samples of suspected gout goslings were collected from Qingyuan,Guangzhou,Jiangmen and Shanwei from 2019 to 2021.Histopathological results showed that there were severe inflammatory cell infiltration and necrosis in liver and kidney of gout-infected goslings,and 72 clinical samples were identified as GoAstV positive by RT-PCR.One positive sample from each of four regions was selected to amplify the whole genome of GoAstV,and four full-length genomic sequences of GoAstV with length of 7033bp were obtained.The results of MegAlign nucleotide homology analysis showed that the nucleotide homology of the whole genome sequence of the four GoAstV strains was 98.4%-99.4%,97.1%-99.7%homology with the reported GoAstV-2 reference strain,and only 57.3%-57.7%homology with GoAstV-1.Compared with other Avastroviruses,GoAstV had the highest homology with turkey astrovirus type 2(TAstV-2),with 65.0%to 65.3%identical to TAstV-2.Molecular feature analysis for the amino acid sequence of capsid(Cap)protein showed that Y^(36)H mutation was identified in the Cap protein of the four identified GoAstV strains.In addition,E^(456)D mutation was also found in the Cap protein of GoAstV/Guangdong/NH2/2019 and GoAstV/Guangdong/GZ-HOD/2021.The genetic evolution analysis of the whole genome of GoAstV and Cap gene showed that GoAstV could be divided into two branches:GoAstV-1 and GoAstV-2,while the four GoAstV strains identified in this study all belonged to GoAstV-2 clade.For the first time,this study revealed the epidemic characteristics and genetic variation of GoAstV in geese in Guangdong,so as to provide reference for the prevention and control of GoAstV and the screening of vaccine strains.

关 键 词：雏鹅痛风 新型鹅星状病毒 遗传演化 变异 

分 类 号：S852.65[农业科学—基础兽医学]