HPLC-DAD法同时测定莪棱胶囊中5种活性成分含量  

Simultaneous Determination of Five Active Compounds in Eleng Capsule by HPLC-DAD

作　　者：邓俊昆 Deng Junkun(Preparation Department,Department of Pharmacy,Guangdong Provincial Hospital of Chinese Medicine,Guangzhou 510170,China)

机构地区：[1]广东省中医院药学部制剂科,广东广州510170

出　　处：《亚太传统医药》2023年第6期49-52,共4页Asia-Pacific Traditional Medicine

摘　　要：目的:建立一种同时测定莪棱胶囊中5种活性成分含量的方法。方法:采用Agilent C18色谱柱(250 mm×4.6 mm,5μm);流动相为乙腈(A)-0.1%磷酸溶液(B),切换检测波长进行梯度洗脱(230 nm,0~15 min,15%A;280 nm,15~16 min,15%A→21%A,16~32 min,21%A,32~45 min,21%A→55%A;270 nm,45~68 min,55%A→85%A);流速1.0 mL/min;柱温30℃;进样体积10μL。结果:莪棱胶囊中5种活性成分在相应浓度范围内线性关系良好(R>0.9990);芍药苷、柚皮苷、橙皮苷、丹酚酸B和丹参酮ⅡA的平均回收率分别为100.53%(RSD=0.41%)、98.91%(RSD=1.26%)、98.56%(RSD=0.53%)、100.03%(RSD=0.93%)和99.54%(RSD=0.99%),精密度、稳定性、重复性良好,RSD值均小于2%。结论:该研究建立的含量测定方法快速、简便、准确,可成为莪棱胶囊质量控制的一种有效方法。Objective:To establish a method for simultaneous determination of five active compounds in Eleng capsule.Methods:Agilent Cis Column(250 mmX 4.6 mm,5μm);Using acetonitrile(A)-0.1%phosphoric acid solution(B)as mobile phase,the detection wavelength was switched for gradient elution(230 nm,0~15 min,15%A;280 nm,15~16 min,15%A→21%A,16~32min,21%A,32~45 min,21%A→55%A;270 nm,45~68 min,55%A→85%A);The flow rate is 1.O mL/min;Column temperature 30 C;The injection volume is 10μL.Results:The five active compounds in Eleng capsule had good linear relationship within the corresponding concentration range(R>0.9990);The average recoveries of paeoniflorin,naringin,hesperidin,salvianolic acid B and tanshinone II^were 100.53%(RSD=0.41%),98.91%(RSD=1.26%),98.56%(RSD=0.53%),100.03%(RSD=0.93%)and 99.54%(RSD=0.99%)respectively.The precision,stability and repeatability were good,and the RSD values were less than 2%.Conclusion:The content determination method established in this study is fast,simple and accurate,and can become an effective method for the quality control of Eleng capsule.

关 键 词：莪棱胶囊 HPLC-DAD 化学成分 

分 类 号：R284.2[医药卫生—中药学]