褪黑素对急性铅中毒小鼠卵母细胞体外成熟质量的影响  

Effect of melatonin on in vitro maturation quality of oocytes in mice with acute lead poisoning

作　　者：牛浩原 瞿静雯 胡慧茹 吴希 李拥军[1] NIU Haoyuan;QU Jingwen;HU Huiru;WU Xi;LI Yongjun(Jiangsu Key Laboratory of Animal Genetics,Breeding and Molecular Design/College of Animal Science and Technology,Yangzhou University,Yangzhou 225009,China)

机构地区：[1]扬州大学动物科学与技术学院/江苏省动物遗传繁育与分子设计重点实验室,江苏扬州225009

出　　处：《扬州大学学报（农业与生命科学版）》2023年第2期68-74,共7页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：江苏现代农业产业技术体系建设专项[JATS(2022)491]。

摘　　要：为探究褪黑素(melatonin)对铅中毒小鼠卵母细胞质量的的影响,以6~8周龄体况相近的ICR雌鼠卵母细胞为研究对象,将其分为5组,分别为生理盐水对照组及4个铅中毒组,并在铅中毒组的卵母细胞体外成熟培养液中分别添加浓度为0、10^(-5)、10^(-7)、10-9mol·L^(-1)的褪黑素,根据第1极体排出率作为体外成熟的标志,筛选出最佳的褪黑素浓度,再检测活性氧(ROS)、谷胱甘肽(GSH)、三磷酸腺苷(ATP)水平,最后通过细胞凋亡试验分析褪黑素对铅中毒卵母细胞体外成熟的影响。结果表明:体外添加1×10^(-7)mol·L^(-1)的褪黑素能显著降低铅中毒小鼠的ROS水平和早期细胞凋亡率,显著提高第1极体排出率及GSH、ATP含量。综上,在培养液中添加1×10^(-7)mol·L^(-1)的褪黑素能在体外成熟过程中显著改善铅中毒雌鼠卵母细胞的质量。To investigate the effects of melatonin on oocyte quality in mice with lead poisoning.Oocytes of 6-8 week-old female ICR mice with similar body conditions were selected as the research objects,and they were divided into 5 groups,one normal saline control group and four lead poisoning groups.The in vitro maturation medium of oocytes of lead poisoning group was supplemented with melatonin at concentrations of 0,10^(-5),10^(-7)and 10^(-9)mol·L^(-1),respectively.According to the excretion rate of the first polar body as a marker of in vitro maturation,the optimal melatonin concentration was screened,and then the reactive oxygen species(ROS),glutathione(GSH)and adenosine triphosphate(ATP)levels were detected.Finally,the effect of melatonin on the in vitro maturation of lead poisoned oocytes was analyzed by apoptosis assay.The results showed that the supplementation of 1×10^(-7)mol·L^(-1)melatonin could significantly reduce the ROS level and early apoptosis rate,and significantly increase the first polar body excretion rate,GSH content and ATP content in mice with lead poisoning.It can be concluded that adding 1×10^(-7)mol·L^(-1)melatonin to the culture medium can significantly improve the quality of lead poisoned female oocytes during in vitro maturation.

关 键 词：卵母细胞 铅中毒 褪黑素 体外成熟 

分 类 号：Q132.1[生物学—普通生物学]