衣康酸酐抗原修复液在改善前处理不佳组织的免疫组织化学染色结果中的应用研究  

Application of itaconic anhydride antigen repair solution in improving immunohistochemical staining results of poorly pretreated tissues

作　　者：陈丽雅 王凤翔 邹琼瑜 廖鸿力 CHEN Liya;WANG Fengxiang;ZOU Qiongyu;LIAO Hongli(Department of Pathology,Wenzhou Central Hospital,Wenzhou 325000,Zhejiang,China)

机构地区：[1]温州市中心医院病理科,浙江温州325000

出　　处：《中国现代医生》2023年第16期67-70,75,共5页China Modern Doctor

基　　金：温州市科研项目(Y20210692)。

摘　　要：目的探讨衣康酸酐抗原修复液改善前处理不佳组织免疫组织化学(以下简称免疫组化)染色结果的效果。方法收集2022年3~5月温州市中心医院术后病理标本68例,其中乳腺癌19例,肠癌32例,扁桃体17例。模拟前处理不佳的组织处理流程制作组织蜡块,每个病例每种标记抗体切片2张,根据随机数字表法分为对照组(使用乙二胺四乙酸抗原修复液)和实验组(使用衣康酸酐抗原修复液),对比两组的免疫组化染色结果。结果通过实验选择4mmol/L的衣康酸酐作为抗原修复液。实验组与对照组的敏感度比较差异无统计学意义(P>0.05);实验组免疫组化染色结果的特异性优良率显著高于对照组(92.48%vs.76.69%,χ^(2)=25.43,P<0.05);实验组免疫组化染色的细胞形态显著优于对照组(χ^(2)=13.60,P<0.01),对照组普遍出现皱褶、局部掉片、细胞肿大甚至严重变形、细胞膜/质/核定位不清晰;而实验组皱褶较少,几乎无掉片现象,细胞形态相对完整,细胞膜/质/核定位相对清晰。结论当组织固定不及时或脱水流程出现问题,建议使用衣康酸酐抗原修复液以获得特异性及细胞形态较好的免疫组化染色结果。Objective To investigate the effect of itaconic anhydride antigen repair solution in improving immunohistochemical staining results of poorly pretreated tissues.Methods From March to May 2022,68 postoperative pathological specimens were collected in Wenzhou Central Hospital,including 19 cases of breast cancer,32 cases of bowel cancer and 17 cases of tonsil.Tissue wax blocks were made by simulating the tissue processing process with poor pretreatment,and of each antibody were made for each case.Two slices of each labeled antibody for each case were devided into control group(using ethylenediaminetetraacetic acid antigen repair solution)and experimental group(using itaconic anhydride antigen repair solution),and compare the immunohistochemical staining results of the two groups.Results Four mmol/L itaconic anhydride was selected as the subsequent antigen repair solution.There was no significant difference in sensitivity between experimental group and control group(P>0.05).The specific excellent and good rate of immunohistochemical staining results in experimental group was significantly higher than that in control group(92.48%vs.76.69%,χ^(2)=25.43,P<0.05).The morphology of cells stained by immunohistochemistry in experimental group was significantly better than that in control group(χ^(2)=13.60,P<0.01).In control group,folds,local sheet loss,cell enlargement or even severe deformation,and unclear cell membrane/plasmic/nuclear localization were common,while in experimental group,folds were less,there was almost no sheet loss,cell morphology was relatively intact,and cell membrane/plasmic/nuclear localization was relatively clear.Conclusion When the tissue is not fixed in time or there is a problem in the dehydration process,it is recommended to use itaconic anhydride antigen repair solution to obtain the specific and better immunohistochemical staining results of cell morphology.

关 键 词：衣康酸酐 免疫组织化学 固定 非特异性结合 

分 类 号：R446.8[医药卫生—诊断学]