基于正交试验的地被小菊组培苗生根培养体系优化  

Optimization of the rooting culture system of Chrysanthemum morifolium tissue culture seedlings based on orthogonal experiment

作　　者：刘晓红 王娅帆 吕婉嘉 郭彦君 杨永青[2] LIU Xiaohong;WANG Yafan;LüWanjia;GUO Yanjun;YANG Yongqing(Department of Art and Design,Taiyuan University,Taiyuan 030032,China;College of Biological Sciences,China Agriculture University,Beijing 100193,China;Jinan Licheng No.2 High School,Taiyuan 030032,China)

机构地区：[1]太原学院艺术设计系,太原030032 [2]中国农业大学生物学院,北京100193 [3]济南市历城第二中学,太原030032

出　　处：《植物生理学报》2023年第4期782-791,共10页Plant Physiology Journal

基　　金：山西省高等学校教学改革创新项目(J2021786);中国农业大学生命科学实验教学示范中心探索项目(20210102和20210103)。

摘　　要：为优化‘千头菊5号’地被小菊组培苗生根技术体系,采用4因素3水平[L_(9)(3~4)]的正交试验法,探究基本培养基、NAA、IBA及活性炭对地被小菊组培苗生根率及生根效果的影响,从而筛选最优的生根培养基。结果表明,不同因素对地被小菊组培苗生根率按重要程度排序如下,(1) 1/2MS>1/4MS>MS;(2) 0.30mg·L^(–1) NAA>0.50 mg·L^(–1) NAA>0 mg·L^(–1) NAA;(3) 0.75 mg·L^(–1) IBA>0.35 mg·L^(–1) IBA>0 mg·L^(–1) IBA;(4)1.00 g·L^(–1) AC=2.00 g·L^(–1) AC>0 g·L^(–1) AC。通过对生根率和其他生根指标的综合评估可得最佳诱导地被小菊生根培养基为1/2MS+0.30 mg·L^(–1) NAA+0.35~0.75 mg·L^(–1) IBA+1.00 g·L^(–1)AC。In order to optimize the rooting technology system of 'Qiantouju5' Chrysanthemum morifolium tissue culture seedlings,the experiment was made based on the design scheme specified through 4-factor 3-level orthogonal test.It is sought to explore the effect on the rooting rate and rooting effect of C.morifolium tissue culture seedlings by each of the following four factors:basic medium,NAA,IBA and activated carbon,so as to select the optimal rooting medium.The results showed that the importance of different factors on the rooting rate of C.morifolium tissue culture seedlings was ranked as follows:(1) 1/2MS1/4MSMS;(2) 0.30 mg·L^(–1)NAA0.50 mg·L^(–1) NAA0 mg·L^(–1) NAA;(3) 0.75 mg·L^(–1) IBA0.35 mg·L^(–1) IBA0 mg·L^(–1) IBA;(4) 1.00 g·L^(–1)AC=2.00 g·L^(–1) AC0 g·L^(–1) AC.Through the comprehensive evaluation of the rooting rate and other rooting indicators,the best induced rooting medium is 1/2MS+0.30 mg·L^(–1) NAA+0.35–0.75 mg·L^(–1) IBA+1.00 g·L^(–1) AC.

关 键 词：‘千头菊5号’地被小菊 组织培养技术 生根培养 正交试验 

分 类 号：S682.11[农业科学—观赏园艺]