β-烟酰胺单核苷酸对反复着床失败患者子宫内膜基质细胞蜕膜化水平的影响  

Influence ofβ-nicotinamide mononucleotide on the decidualization level of human endometrial stromal cells of patients with repeated implantation failure

作　　者：韩烁 时晓 鹿群 刘明慧[1] HAN Shuo;SHI Xiao;LU Qun;LIU Minghui(Beijing Chao-yang Hospital,Capital Medical University,Beijing,100020)

机构地区：[1]首都医科大学附属北京朝阳医院,100020

出　　处：《中国计划生育学杂志》2023年第6期1276-1280,1498,共6页Chinese Journal of Family Planning

基　　金：首都医科大学科研培育基金(118208150802)。

摘　　要：目的:探讨在体外受精-胚胎移植反复着床失败患者子宫内膜基质细胞中添加β-烟酰胺单核苷酸(β-NMN)对子宫内膜基质细胞蜕膜化水平的影响。方法:活检获得反复着床失败3例患者子宫内膜组织,酶消化分离体外培养后获得了三株原代子宫内膜基质细胞。根据培养过程中添加β-NMN和诱导蜕膜化情况分为实验组、对照组、空白组。实验组在子宫内膜基质细胞蜕膜化培养过程中添加β-NMN,对照组不添加β-NMN,无蜕膜化培养为空白组。比较3组细胞培养过程中蜕膜标志物泌乳素(PRL)和人胰岛素样生长因子结合蛋白1(IGFBP-1)的mRNA表达和分泌水平。结果:实验组PRL(2.38±0.10 ng/nl)、IGFBP-1(1.89±0.12 ng/nl)水平均高于对照组(1.19±0.17 ng/nl、0.99±0.20 ng/nl)(P<0.05),对照组与空白组相比无差异;定量PCR检测蜕膜前后细胞内PRL(15.82±12.92)和IGFBP-1(9.86±8.67)的mRNA表达量上升(P<0.05)。结论:反复着床失败患者子宫内膜基质细胞蜕膜化中添加β-NMN能够提高蜕膜化水平。Objective:To investigate the influence ofβ-nicotinamide mononucleotide(β-NMN)on the decidualization level of human endometrial stromal cells of patients with repeated implantation failure.Methods:Endometrial tissues from 3patients with repeated implantation failure were obtained by endometrial biopsy,and three primary human endometrial stromal cells were isolated from endometrial tissues after enzyme digestion and cultured in vitro.According to whetherβ-NMN added and the decidualization of the endometrial stromal cells,these cells were divided into experimental group,control group,and blank group.In the experimental group,β-NMN was added during decidualization of endometrial stromal cells.In the control group,β-NMN was not added during decidualization cultured of endometrial stromal cells.In the blank group,the endometrial stromal cells had not been decidualization cultured.The levels of the mRNA expression and the secretion of the decidual markers,such as prolactin(PRL)and human insulin-like growth factor binding protein 1(IGFBP-1)of the cells during cultured were compared among the three groups.Results:The levels of PRL(2.38±0.10ng/nl)and IGFBP-1(1.89±0.12ng/nl)of the cells in the experimental group were significantly higher than those(1.19±0.17ng/nl and 0.99±0.20ng/nl)of the cells in the control group(P<0.05),and which of the cells in the control group had no significant different from those of the cells in the blank group.The mRNA expression levels of PRL(15.82±12.92)and IGFBP-1(9.86±8.67)in the cells detected by quantitative PCR had increased after decidualization cultured significantly(P<0.05).Conclusion:β-NMN added in the human endometrial stromal cells during decidualization cultured of the patients with repeated implantation failure can improve the decidualization level of these cells.

关 键 词：体外受精-胚胎移植 反复着床失败 子宫内膜基质细胞 β-烟酰胺单核苷酸 蜕膜化 泌乳素 人胰岛素样生长因子结合蛋白1 

分 类 号：R714.8[医药卫生—妇产科学]