妊娠期高血压患者血清lncRNA FAM99A与不良妊娠结局  

Expression of serum long non coding RNA family with sequence similarity 99 member A of women with hypertensive disorders complicating pregnancy and its associated with the adverse pregnancy outcomes

作　　者：魏伟[1] 张婷[1] 陶玲玲 WEI Wei;ZHANG Ting;TAO Lingling(The Third Affiliated Hospital of Zhengzhou University,Zhengzhou,Henan Province,450052)

机构地区：[1]郑州大学第三附属医院,郑州450052

出　　处：《中国计划生育学杂志》2023年第6期1420-1424,共5页Chinese Journal of Family Planning

摘　　要：目的:探究长链非编码RNA家族成员A序列相似性99(lncRNA FAM99A)在妊娠期高血压(HDCP)患者血清中的表达及其与妊娠结局的相关性。方法:收集2019年9月-2021年9月本院接诊的HDCP患者120例临床资料,其中妊娠期高血压组40例、轻度子痫前期组29例、重度子痫前期组51例,正常产前检查孕妇120例为对照组,收集基线资料,荧光定量PCR法检测血清lncRNA FAM99A水平;Spearman相关分析法分析lncRNA FAM99A表达与妊娠结局的相关性,根据HDCP患者妊娠结局分为妊娠结局不良组(33例)和妊娠结局良好组(87例),绘制受术者工作特征曲线评价lncRNA FAM99A水平对HDCP孕妇不良妊娠结局的预测价值。结果:随着HDCP病情加重lncRNA FAM99A表达降低,有指征的剖宫产、产后出血、早产或过期产、新生儿窒息发生率HDCP组高于对照组(均P<0.05);HDCP患者妊娠结局不良组血清lncRNA FAM99A(0.34±0.09)低于妊娠结局良好组(0.77±0.20)(P<0.05);血清lncRNA FAM99A预测HDCP患者不良妊娠结局的曲线下面积为0.797,截断值0.61,灵敏度84.9%、特异性70.1%。结论:HDCP患者血清lncRNA FAM99A表达下调,下调的lncRNA FAM99A与不良妊娠结局有关。Objective:To investigate the expression of long non coding RNA family with sequence similarity 99member A(lncRNA FAM99A)of pregnant women with hypertensive disorders complicating pregnancy(HDCP),and to study its associated with the pregnancy outcomes of these women.Methods:The clinical data of 120pregnant women with HDCP from September 2019to September 2021were collected in study group.Among them,there were 40women with HDCP in group A,29women with mild preeclampsia in group B,and 51women with severe preeclampsia in group C.Another 120normal pregnant women were selected in control group during the same period.The baseline data of the women in these groups were collected.The level of serum lncRNA FAM99Awas detected by fluorescence quantitative PCR.The correlation between the expression level of lncRNA FAM99Aof the women and their pregnancy outcomes was analyzed by Spearman correlation analysis.According to the pregnancy outcomes of the women with HDCP,the women in the study groups were divided into group D(87cases with normal pregnancy outcomes)and group E(33cases with adverse pregnancy outcomes).Receiver operating characteristic(ROC)curve was drawn to evaluate the predictive value of lncRNA FAM99Alevel of the women with HDCP for their adverse pregnancy outcomes.Results:The lncRNA FAM99Aexpression level of the women had decreased with the aggravation of their HDCP.The rates of the cesarean section due to the indications,the postpartum hemorrhage,the premature birth or post-term birth,and the neonatal asphyxia of the women in the study group were significantly higher than those of the women in the control group(all P<0.05).The serum lncRNA FAM99Alevel(0.34±0.09)of the women in group E was significantly lower than that(0.77±0.20)of the women in group D(P<0.05).The area under curve,the cut-off value,the sensitivity,and the specificity of the serum lncRNA FAM99Aof the women with HDCP for predicting their adverse pregnancy outcomes were 0.797,0.61,84.9%,and 70.1%,respectively.Conclusion:The lncRNA FAM99A expression o

关 键 词：妊娠期高血压 长链非编码RNA家族成员A序列相似性99 病情进展 相关性 不良妊娠结局 预测 

分 类 号：R714.246[医药卫生—妇产科学]