膀胱癌组织中miR-144、肺癌转移相关转录本1及睾丸蛋白聚糖表达及其诊断价值  

Expression and diagnostic value of miR-144,MALAT1 and SPOCK1 in bladder cancer tissue

作　　者：梁博[1] 何跃[1] 刘泓键 奉友刚[1] 何俊[1] LIANG Bo;HE Yue;LIU Hongjian(Department of Urology,Suining Central Hospital,Sichuan,Suining 629000,China)

机构地区：[1]四川省遂宁市中心医院泌尿外科,629000

出　　处：《临床外科杂志》2023年第5期440-443,共4页Journal of Clinical Surgery

摘　　要：目的分析膀胱癌组织中miR-144、肺癌转移相关转录本1(MALA T1)及睾丸蛋白聚糖(SPOCK1)表达及其诊断价值。方法2018年1月~2021年1月本院收治的膀胱尿路上皮癌病人96例,膀胱电切或膀胱根治性切除留取膀胱癌病人新鲜肿瘤组织(肿瘤组)及距离肿瘤边缘>2cm癌旁正常组织(癌旁组)标本,各5mg。使用聚合酶链式反应(PCR)检测不同组织中miR-144、MALAT1及SPOCK1相对表达量,分析miR-144、MALAT1及SPOCK1在不同膀胱癌病理特征中表达情况,绘制ROC曲线,检验miR-144、MALAT1、SPOCK1对膀胱癌的诊断价值。96例病人均在本院进行外科手术+术后辅助化疗,通过电话和门诊随访等方式对病人进行随访,随访截止至2022年1月30日,比较不同预后者miR-144、MALAT1及SPOCK1的表达。结果肿瘤组中MALAT1及SPOCK1相对表达量高于癌旁组,而miR-144相对表达量则低于癌旁组,差异有统计学意义(P<0.05)。Ⅲ~Ⅳ期、有淋巴结转移者miR-144表达低于Ⅰ~Ⅱ期、无淋巴结转移者,差异有统计学意义(P<0.05),Ⅲ~Ⅳ期、低分化、有淋巴结转移者MALAT1、SPOCK表达高于Ⅰ~Ⅱ期、中高分化与无淋巴结转移者,差异有统计学意义(P<0.05)。miR-144、MALAT1及SPOCK1对膀胱癌诊断AUC值分别为0.643、0.557,SPOCK1诊断AUC值为0.717(95%Cl:0.688~0.845),以SPOCK1诊断AUC值最高(P<0.05)。96例病人预后良好85例,预后不良11例。预后不良组miR-144低于预后良好组,MALAT1及SPOCK1高于预后良好组,差异有统计学意义(P<0.05)。结论膀胱癌组织中miR-144、MALAT1及SPOCK1表达异常,与病人病理特征存在一定联系,或可作为膀胱癌潜在诊断标志物。Objective To analyze the expression of miR-144,lung cancer metastasis-associated transcript 1(MALA T1)and testis proteoglycan(SPOCK1)in bladder cancer tissues and their diagnostic efficacy.Methods A total of 96 bladder cancer patients who were treated in our hospital from January 2018 to January 2021 were selected.Fresh tumor tissue from bladder cancer patients(tumor group)and normal tissue adjacent to the tumor>2 cm away from the tumor edge(paracancerous group)were collected during surgery.5 mg each.The relative expression levels of miR-144,MALAT1 and SPOCK1 in different tissues were detected by polymerase chain reaction(PCR),and the expressions of miR-144,MALAT1 and SPOCK1 in different pathological features of bladder cancer were analyzed.The ROC curve was drawn to test the diagnostic effect of miR-144,MALAT1 and SPOCK1 on bladder cancer.96 patients underwent surgery+postoperative adjuvant chemotherapy in our hospital,and the patients were followed up by telephone and outpatient followup.Followup ended on January 30,2022,and the expressions of miR-144,MALAT1 and SPOCK1 were compared in patients with different prognosis.Results The relative expressions of MALAT1 and SPOCK1 in the tumor group were significantly higher than those in the paracancerous group,while the relative expression of miR-144 was significantly lower than that in the control group(P<0.05).The expression of miR-144 in stageⅢ-Ⅳpatients with lymph node metastasis was significantly lower than that in stageⅠ-Ⅱpatients without lymph node metastasis(P<0.05).MALAT1 and SPOCK levels in stageⅢ-Ⅳ,poorly differentiated,and lymph node metastasis were significantly higher than those in stageⅠ-Ⅱ,moderately and well differentiated,and no lymph node metastasis(P<0.05).The ROC curve was drawn,and the AUC value was calculated.The AUC values of miR-144,MALAT1 and SPOCK1 for the diagnosis of bladder cancer were 0.643 and 0.557,respectively,and the AUC value for the diagnosis of SPOCK1 was 0.717(95%Cl:0.688-0.845).the highest AUC value was diagnosed

关 键 词：膀胱癌 miR-144 肺癌转移相关转录本1 睾丸蛋白聚糖 分化程度 病理特征 

分 类 号：R737.14[医药卫生—肿瘤]