血管活性肠多肽对老龄勃起功能障碍大鼠的作用及机制研究  

The effects of vasoactive intestinal polypeptide on erectile dysfunction in aged rats and the underlying mechanism

作　　者：张存明 王军卫 陈松 吴忠标 叶海波 ZHANG Cun-ming;WANG Jun-wei;CHEN Song;WU Zhong-biao;YE Hai-bo(Department of Urology,The Affiliated Wenling Hospital of Wenzhou Medical University,Wenling,Zhejiang 317500,China)

机构地区：[1]温州医科大学附属温岭医院泌尿外科,温岭317500

出　　处：《浙江中西医结合杂志》2023年第6期497-501,共5页Zhejiang Journal of Integrated Traditional Chinese and Western Medicine

基　　金：浙江省温岭市科技计划项目(No.2018C310012)。

摘　　要：目的 观察血管活性肠多肽(VIP)对老龄勃起功能障碍(ED)大鼠的作用及其机制。方法40只SPF级雄性SD大鼠饲养至18月龄构建老龄模型大鼠,采用阿扑吗啡实验筛选老龄ED大鼠,阿扑吗啡实验结果阳性的老龄勃起功能正常大鼠为正常组,实验结果阴性的老龄ED大鼠为ED组和干预组,每组7只。干预组大鼠使用VIP 25 ng/kg隔日腹腔注射,治疗28 d。检测阴茎海绵体内压(ICP)及平均动脉压(MAP)评估勃起功能;酶联免疫吸附法检测大鼠阴茎海绵体组织环磷酸腺苷(cAMP)、一氧化氮(NO)含量;组织免疫荧光技术(IF)测大鼠阴茎海绵体组织血管性血友病因子(vWF)表达水平;蛋白质印迹法检测海绵体蛋白激酶A (PKA)、内皮源性一氧化氮合酶(eNOS)、血管性血友病因子(vWF)及血管内皮生长因子(VEGF)蛋白表达水平。结果 正常组、ED组和干预组基础ICP分别为(20.41±5.92)mmHg、(21.76±5.37)mmHg和(18.54±3.97)mmHg(1mmHg=0.133 kPa),MAP分别为(123.52±14.74)mmHg,(118.83±10.97)mmHg和(114.28±12.21)mmHg,各组间比较,差异无统计学意义(P>0.05)。与正常组比较,ED组Max ICP、Max ICP/MAP明显降低[Max ICP:(42.10±6.57)mmHg比(94.82±9.71)mmHg;Max ICP/MAP:(0.36±0.08)比(0.78±0.16),P均<0.01]。与ED组比较,干预组Max ICP、Max ICP/MAP明显升高[Max ICP:(59.52±2.20)mmHg比(42.10±6.57)mmHg,P<0.01;Max ICP/MAP:(0.52±0.06)比(0.36±0.08),P<0.05]。与正常组比较,ED组vWF荧光强度明显减少,干预组明显增加。与正常组比较,ED组cAMP和NO含量均降低[cAMP:(33.11±11.82)pmol/mg比(94.63±9.36)pmol/mg;NO:(3.05±1.51)nmol/mg比(11.59±2.43) nmol/mg,P均<0.01];PKA、eNOS、vWF及VEGF蛋白表达水平降低[PKA:(0.66±0.08)比(1.04±0.22);eNOS:(0.54±0.13)比(0.71±0.09);vWF:(1.22±0.30)比(2.04±0.24);VEGF:(0.45±0.13)比(0.82±0.18),P<0.05或P<0.01];与ED组比较,干预组cAMP、NO含量均增加[cAMP:(58.46±9.48)pmol/mg比(33.11±11.82)pmol/mg;NO:(5.31±1.39)nmol/mg比(3.05±1.51)nmol/mg,P<0.05或P<0.01];PKA、eObjective To observe the effect of vasoactive intestinal polypeptide on the aging rat model of erectile dysfunction and the potential underlying mechanism.Methods An apomorphine feeding study on 18-month-old rats was used to screen for erectile dysfunction(ED)model.The aged rats with normal erectile function and positive apomorphine test results served as the control group,while the aged ED rats with negative test results served as the ED group or the intervention group,with seven rats in each group.The aged ED rats in the experimental group received intraperitoneal injection of 25 ng/kg of vasoactive intestinal polypeptide every other day for 28 days.Intracavernosal pressure(ICP)and mean arterial pressure(MAP)were used to assess erectile function.Enzyme-linked immunosorbent assay was used to detect cAMP and NO content.Immunofluorescence assay was used to detect the expression level of vWF in corpora cavernosa tissue.Western blot was used to detect protein kinase A(PKA),endothelial nitric oxide synthase(eNOS),von Willebrand factor(vWF),and vascular endothelial growth factor(VEGF)protein expression levels in corpus cavernosa.Results The basal ICP was 20.41±5.92,21.76±5.37,and 18.54±3.97 mmHg,and MAP was 123.5±14.74,118.8±10.97,and 114.2±12.21 mmHg in the normal,model and experimental groups respectively,with no statistical difference between the groups(P>0.05).Compared with the normal group,both Max ICP and Max ICP/MAP in the ED group were significantly reduced(Max ICP:42.10±6.57 mmHg vs.94.82±9.71 mmHg;Max ICP/MAP:0.36±0.08 vs.0.78±0.16,P<0.01).Conversely,in comparison to the ED group,the intervention group showed a significant increase in both Max ICP and Max ICP/MAP(Max ICP:59.52±2.20 mmHg vs.42.10±6.57 mmHg,P<0.01;Max ICP/MAP:0.52±0.06 vs.0.36±0.08,P<0.05).Furthermore,the vWF fluorescence intensity was significantly decreased in the ED group compared to the normal group,whereas it was markedly increased in the intervention group.Compared with the normal group,both cAMP and NO levels were signific

关 键 词：大鼠 血管活性肠多肽 环磷酸腺苷 蛋白激酶A 勃起功能障碍 老年 

分 类 号：R698[医药卫生—泌尿科学]