An enzyme cascade enables production of therapeutic oligonucleotides in a single operation  

作　　者：E R Moody 

机构地区：[1]不详

出　　处：《四川生理科学杂志》2023年第6期1087-1087,共1页Sichuan Journal of Physiological Sciences

摘　　要：Therapeutic oligonucleotides have emerged as a powerful drug modality with the potential to treat a wide range of diseases;however,the rising number of therapies poses a manufacturing challenge.Existing synthetic methods use stepwise extension of sequences immobilized on solid supports and are limited by their scalability and sustainability.We report a biocatalytic approach to efficiently produce oligonucleotides in a single operation where polymerases and endonucleases work in synergy to amplify complementary sequences embedded within catalytic self-priming templates.This approach uses unprotected building blocks and aqueous conditions.We demonstrate the versatility of this methodology through the synthesis of clinically relevant oligonucleotide sequences containing diverse modifications.

关 键 词：enable COMPLEMENTARY STEPWISE 

分 类 号：O64[理学—物理化学]