纳米酶合成及其传感的分析化学综合设计实验  

Comprehensive Design Experiment of Analytical Chemistry Based on Synthesis and Sensing applications of Nanoenzymes

作　　者：叶晓生 黄胜 Ye Xiaosheng;Huang Sheng(School of Resource and Environment,Hunan University of Technology and Business,Changsha 410205;Higher Education Research Institute,Hunan University of Technology and Business,Changsha 410205,China)

机构地区：[1]湖南工商大学资源环境学院,湖南长沙410205 [2]湖南工商大学高等教育研究院,湖南长沙410205

出　　处：《广东化工》2023年第11期235-237,共3页Guangdong Chemical Industry

基　　金：湖南省社科评审委项目:后疫情时代高校生命教育的现实重审与优化路径研究(XSP22YBC507);追寻生命的整全:大学生心理健康现状及干预路径实证研究(校教字[2021]22号)。

摘　　要：设计了一个氧化还原法制备纳米酶,并进一步将其应用于传感的本科生分析化学综合设计实验。首先,采用了一种简便、温和、一锅煮的方法制备了复合金属纳米酶(Cu/Au/Pt NPs);随后对其大小、形貌及拟酶催化性能进行了表征;最后利用其催化性能实现了对双氧水与葡萄糖的检测应用。本实验通过纳米酶的制备、表征及其分析应用,可以锻炼、激发学生自主学习的内在动力,培养学生理性思维、团队协作和综合实践能力。The nanoenzyme was fabricated by a redox method and applied for sensing,which was introduced to comprehensive design experiments of analytical chemistry for undergraduate students.Firstly,a simple,mild and one-pot way was used to prepare composite metal nano enzyme(Cu/Au/Pt NPs).Then,the features of Cu/Au/Pt NPs were systematically characterized including size,morphology and enzyme-like catalytic activity.Finally,detection of hydrogen peroxide and glucose were performed based on the catalytic performance.Through this experiment,it would make sense to exercise and stimulate the internal motivation of students'independent learning,and cultivate students'rational thinking,teamwork and comprehensive practical ability.

关 键 词：纳米酶 综合设计性实验 实验教学改革 人才培养 创新 

分 类 号：TQ[化学工程]