淫羊藿多糖组成分析及体外抗氧化抗肾癌活性研究  

Composition Analysis,Antioxidant and Anti-renal-cancer Activities of Epimedium Polysaccharide

作　　者：刘坤 滕仕杰 郝亮 于守君 Liu Kun;Teng Shijie;Hao Liang;Yu Shoujun(Yantai Affiliated Hospital of Binzhou Medical University,Yantai 264100;Yantai Traditional Chinese Medicine Hospital,Yantai 264100;Jinling Town Health Center,Zhaoyuan 265400,China)

机构地区：[1]滨州医学院烟台附属医院,山东烟台264100 [2]烟台市中医医院,山东烟台264100 [3]金岭镇卫生院,山东招远265400

出　　处：《广东化工》2023年第12期56-59,共4页Guangdong Chemical Industry

基　　金：山东省重点研发计划山东省重点研发计划(软科学项目)(2018GSF118131);山东省中医药发展项目(2017-237)。

摘　　要：以中药材淫羊藿为材料,采用水提醇沉法,获得淫羊藿中多糖类成分,采用PMP柱前衍生化HPLC方法,对淫羊藿多糖进行单糖组成分析,利用DPPH和ABTS方法对其进行抗氧化活性的研究,采用CCK8法确定其对肾癌细胞活力的影响,细胞划痕实验分析其对肾癌细胞迁移能力的影响,q RT-PCR分析其对肾癌细胞p21通路关键基因表达的影响。结果显示淫羊藿多糖主要由甘露糖、鼠李糖、半乳糖醛酸、葡萄糖、半乳糖、阿拉伯糖6种单糖组成,其DPPH和ABTS抗氧化自由基清除率分别为84.7%和75.6%。淫羊藿多糖能够显著性降低肾癌786-O肾癌细胞活力和迁移能力,显著性提高p21基因的表达,进而抑制CDK1、CDK2和CDK4基因的表达。综上淫羊藿多糖具有良好的抗肾癌和抗氧化活性。The polysaccharides in Epimedium were extracted by water extraction and alcohol precipitation.The monosaccharide composition of Epimedium polysaccharide was analyzed by PMP pre-column derivatization HPLC.The antioxidant activity was evaluated by DPPH and ABTS methods.CCK8 assay and cell scratched assay were used to determine the effect of Epimedium polysaccharide on the viability of the migration of renal cancer cells,respectively.QRT-PCR was used to analyze its effect on the expression of key genes in the p21 pathway of renal cancer cells.The results showed that Epimedium polysaccharide was mainly composed of 6 monosaccharides such as mannose,rhamnose,galacturonic acid,glucose,galactose and arabinose.The antioxidant free radical scavenging rates of DPPH and ABTS were 84.7% and 75.6%,respectively.Epimedium polysaccharide can significantly reduce the viability and migration of 786-O cell.Finally,qRT-PCR results showed that Epimedium polysaccharide could significantly increase the expression of p21 gene and inhibit the expression of CDK1,CDK2 and CDK4.Epimedium polysaccharide has good antioxidant and anti-renal-cancer activities.

关 键 词：淫羊藿 多糖 抗氧化 肾癌 P21 

分 类 号：Q279[生物学—细胞生物学] R285.5[医药卫生—中药学]