RP-HPLC法测定眩晕宁颗粒中3,5-O-二咖啡酰基奎宁酸、23-乙酰泽泻醇B和β-蜕皮甾酮  

Determination of 3,5-O-dicaffeoyl quinic acid,23-acetylalisol B,andβ-ecdysterone in Xuanyunning Granules by RP-HPLC

作　　者：王泳 汪燕[1] 黄灿[1] 陶贤琦 邢薇薇 齐腊梅[1] WANG Yong;WANG Yan;HUANG Can;TAO Xian-qi;XING Wei-wei;QI La-mei(Department of Pharmacy Management,Anqing Municipal Hospital,Anqing 246003,China;Anhui Institute for Food and Drug Inspection,Hefei 230051,China)

机构地区：[1]安庆市立医院药事管理科,安徽安庆246003 [2]安徽省食品药品检验研究院,安徽合肥230051

出　　处：《现代药物与临床》2023年第5期1109-1112,共4页Drugs & Clinic

基　　金：安徽医科大学校科研基金项目(2020xkj241)。

摘　　要：目的建立RP-HPLC法测定眩晕宁颗粒中3,5-O-二咖啡酰基奎宁酸、23-乙酰泽泻醇B和β-蜕皮甾酮的测定方法。方法采用Capcell Pak UG C18色谱柱(250 mm×4.6 mm,5μm);流动相为乙腈–0.5%磷酸溶液,梯度洗脱;检测波长:348 nm(3,5-O-二咖啡酰基奎宁酸)、250 nm(23-乙酰泽泻醇B)、208 nm(β-蜕皮甾酮);体积流量:1.0 mL/min;柱温:28℃;进样量:10μL。结果3,5-O-二咖啡酰基奎宁酸、β-蜕皮甾酮和23-乙酰泽泻醇B分别在0.0767~7.6700、0.0983~9.8300、0.0197~1.9700μg/mL线性良好,平均回收率分别为100.05%、98.33%、99.42%,RSD值分别为0.7%、1.2%、1.3%。结论方法具有前处理简单、分析时间短、检测结果准确等优点,适用于眩晕宁颗粒的质量控制。Objective To establish an RP-HPLC method for the determination of 3,5-O-dicaffeyl quinic acid,23-acetylalisol B,andβ-ecdysteroid in Xuanyunning Granules.Methods The separation was carried out on Capcell Pak UG C18 column(250 mm×4.6 mm,5μm).The mobile phase was acetonitrile-0.5%phosphoric acid solution with gradient elution.The detection wavelength was set at 348 nm(3,5-O-dicaffeyl quinic acid),250 nm(23-acetylalisol B),and 208 nm(β-ecdysterone).The flow rate was 1.0 mL/min,temperature of column was set at 28℃,and the volume of injection was 10μL.Results 3,5-O-dicaffeyl quinic acid,23-acetylalisol B,andβ-ecdysteroid showed good linear relationships in concentration ranges of 0.0767—7.6700,0.0983—9.8300,and 0.0197—1.9700μg/mL,and their average recoveries were 100.05%,98.33%,and 99.42%with RSD values of 0.7%,1.2%,and 1.3%,respectively.Conclusion The method has the advantages of simple pretreatment,short analysis time,and accurate detection results,and is suitable for the quality control of Xuanyunning Granules.

关 键 词：眩晕宁颗粒 3 5-O-二咖啡酰基奎宁酸 23-乙酰泽泻醇B β-蜕皮甾酮 RP-HPLC 

分 类 号：R286.02[医药卫生—中药学]