Revealing CO_(2)-Fixing SAR11 Bacteria in the Ocean by Raman-Based Single-Cell Metabolic Profiling and Genomics  

作　　者：Xiaoyan Jing Yanhai Gong Teng Xu Paul ADavison Craig MacGregor-Chatwin CNeil Hunter La Xu Yu Meng Yuetong Ji Bo Ma Jian Xu Wei E.Huang 

机构地区：[1]Single-Cell Center,CAS Key Laboratory of Biofuels,Shandong Key Laboratory of Energy Genetics and Shandong Institute of Energy Research,Qingdao Institute of Bioenergy and Bioprocess Technology,Chinese Academy of Sciences,Qingdao,Shandong,China [2]Laboratory for Marine Biology and Biotechnology,Qingdao National Laboratory for Marine Science and Technology,Qingdao,Shandong,China [3]University of Chinese Academy of Sciences,Beijing,China [4]Plants,Photosynthesis and Soil,School of Biosciences,University of Sheffield,Sheffield S102TN,UK [5]Disease and Fishery Drugs Research Center,Marine Biology Institute of Shandong Province,Qingdao,Shandong,China [6]Single-Cell Biotechnology,Ltd,Qingdao,Shandong,China [7]Department of Engineering Science,University of Oxford,Parks Road,OX13PJ Oxford,UK

出　　处：《BioDesign Research》2022年第1期52-67,共16页生物设计研究（英文）

基　　金：the National Key R&D Program Young Scientists Project of China(No.2021YFD1900400 to XYJ);the National Science Foundation of China(No.32270109 to XYJ);BM acknowledges the support of the Key Instrument Development Program from Chinese Academy of Sciences(YJKYYQ20170017);WEH acknowledges finance and instrumentation support from the Engineering and Physical Sciences Research Council(EP/M002403/1 and EP/M02833X/1)and Natural Environment Research Council(NE/M002934/1);WEH,PAD,CMC,and CNH acknowledge financial support from the Biotechnology and Biological Sciences Research Council(BBSRC UK),award number BB/M000265/1;CNH and PAD are supported by the European Research Council Synergy Award 854126.We also thank NSFC(31770112)for the financial support.

摘　　要：The majority of marine microbes remain uncultured,which hinders the identification and mining of CO_(2)-fixing genes,pathways,and chassis from the oceans.Here,we investigated CO_(2)-fixing microbes in seawater from the euphotic zone of the Yellow Sea of China by detecting and tracking their ^(13)C-bicarbonate(^(13)C-HCO_(3)^(-))intake via single-cell Raman spectra(SCRS)analysis.The target cells were then isolated by Raman-activated Gravity-driven Encapsulation(RAGE),and their genomes were amplified and sequenced at one-cell resolution.The single-cell metabolism,phenotype and genome are consistent.We identified a notyet-cultured Pelagibacter spp.,which actively assimilates ^(13)C-HCO_(3)^(-),and also possesses most of the genes encoding enzymes of the Calvin-Benson cycle for CO_(2) fixation,a complete gene set for a rhodopsin-based light-harvesting system,and the full genes necessary for carotenoid synthesis.The four proteorhodopsin(PR)genes identified in the Pelagibacter spp.were confirmed by heterologous expression in E.coli.These results suggest that hitherto uncultured Pelagibacter spp.uses light-powered metabolism to contribute to global carbon cycling.

关 键 词：metabolism BENSON hinder 

分 类 号：Q943.2[生物学—植物学]