Stoichiometric Conversion of Maltose for Biomanufacturing by In Vitro Synthetic Enzymatic Biosystems  

作　　者：Guowei Li Xinlei Wei Ranran Wu Wei Zhou Yunjie Li Zhiguang Zhu Chun You 

机构地区：[1]Tianjin Institute of Industrial Biotechnology,Chinese Academy of Sciences,32 West 7th Avenue,Tianjin Airport Economic Area,Tianjin 300308,China [2]College of Biotechnology,Tianjin University of Science and Technology,1038 Dagu Nanlu,Hexi District,Tianjin 300457,China [3]National Technology Innovation Center of Synthetic Biology,Tianjin 300308,China

出　　处：《BioDesign Research》2022年第1期217-227,共11页生物设计研究（英文）

基　　金：the National Key Research and Development Program of China(Grant number 2021YFA0910601);the National Natural Science Foundation of China(Grant numbers 32022044 and 32001027).

摘　　要：Maltose is a natural α-(1,4)-linked disaccharide with wide applications in food industries and microbial fermentation. However,maltose has scarcely been used for in vitro biosynthesis, possibly because its phosphorylation by maltose phosphorylase (MP)yields β-glucose 1-phosphate (β-G1P) that cannot be utilized by α-phosphoglucomutase (α-PGM) commonly found in in vitrosynthetic enzymatic biosystems previously constructed by our group. Herein, we designed an in vitro synthetic enzymaticreaction module comprised of MP, β-phosphoglucomutase (β-PGM), and polyphosphate glucokinase (PPGK) for thestoichiometric conversion of each maltose molecule to two glucose 6-phosphate (G6P) molecules. Based on this syntheticmodule, we further constructed two in vitro synthetic biosystems to produce bioelectricity and fructose 1,6-diphosphate (FDP),respectively. The 14-enzyme biobattery achieved a Faraday efficiency of 96.4% and a maximal power density of 0.6mW/cm^(2),whereas the 5-enzyme in vitro FDP-producing biosystem yielded 187.0mM FDP from 50 g/L (139mM) maltose by adopting afed-batch substrate feeding strategy. Our study not only suggests new application scenarios for maltose but also provides novelstrategies for the high-efficient production of bioelectricity and value-added biochemicals.

关 键 词：ENZYMATIC utilized MAXIMAL 

分 类 号：O62[理学—有机化学]