Polyphenol based hybrid nano-aggregates modified collagen fibers of biological valve leaflets to achieve enhanced mechanical,anticoagulation and anti-calcification properties  

作　　者：Shufen Li Shiying Lang Zhiqian Chen Jingruo Chen Weihua Zhuang Yangrui Du Yawen Yao Gongyan Liu Mao Chen 

机构地区：[1]Laboratory of Heart Valve Disease,West China Hospital,Sichuan University,37 Guoxue Alley,Chengdu 610041,China [2]The Key Laboratory of Leather Chemistry and Engineering of Ministry of Education,College of Biomass Science and Engineering,Sichuan University,Chengdu 610065,China [3]University of California Davis,1 Shields Ave,Davis,CA 95616,USA [4]Faculty of Science and Engineering,Aachen-Maastricht Institute for Biobased Materials(AMIBM),Maastricht University,Brightlands Chemelot Campus,6167 RD Geleen,The Netherlands [5]Department of Cardiology,West China Hospital,Sichuan University,37 Guoxue Alley,Chengdu 610041,China [6]Regenerative Medicine Research Center,West China Hospital,Sichuan University,37 Guoxue Alley,Chengdu 610041,China

出　　处：《Journal of Leather Science and Engineering》2022年第1期394-408,共15页皮革科学与工程（英文）

基　　金：the National Natural Science Foundation of China(81970325 and 82170375);Key Research and Develop-ment Support Project of Science&Technology Department of Chengdu(2021-YF08-00121-GX);Post-Doctor Research Project,West China Hospital,Sichuan University(Grant No.20HXBH164 and 20HXBH105);Chinese Medical Association Cardiovascular Branch(CSC)Clinical Research Special Fund Project(CSCF2020B04);West China Hospital“1·3·5”Discipline of Excellence Project-“Percutaneous transcatheter aortic valve implantation”and“Mechanisms of aortic stenosis and the clinical applications”(ZYGD20002).

摘　　要：Glutaraldehyde(Glut)-crosslinked porcine pericardium and bovine pericardium are mainly consisted of collagen and widely used for the preparation of heterogenous bioprosthetic heart valves(BHV),which play an important role in the replacement therapy of severe valvular heart disease,while their durability is limited by degeneration due to calcification,thrombus,endothelialization difficulty and prosthetic valve endocarditis.Herein,we develop a novel BHV,namely,TPly-BP,based on natural tannic acid and polylysine to improve the durability of Glut crosslinked bovine pericardium(Glut-BP).Impressively,tannic acid and polylysine could form nanoaggregates via multiple hydrogen bonds and covalent bonds,and the introduction of nanoaggregates not only improved the mechanical properties and collagen stability but also endowed TPly-BP with good biocompatibility and hemocompatibility.Compared to Glut-BP,TPly-BP showed significantly reduced cytotoxicity,improved endothelial cell adhesion,a low hemolysis ratio and obviously reduced platelet adhesion.Importantly,TPly-BP exhibited great antibacterial and in vivo anti-calcification ability,which was expected to improve the in vivo durability of BHVs.These results suggested that TPly-BP would be a potential candidate for BHV.

关 键 词：Tannic acid Bioprosthetic heart valve Antibacterial and anti-calcification 

分 类 号：R318.08[医药卫生—生物医学工程]