A novel domain-duplicated SlitFAR3 gene involved in sex pheromone biosynthesis in Spodoptera litura  

作　　者：Biyun Zhang Fengqi Li Cheng Qu Hongxia Duan Yuejun Fu Chen Luo 

机构地区：[1]Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education,Institute of Biotechnology,Shanxi University,Taiyuan,China [2]Institute of Plant Protection,Bejing Academy of Agriculture and Forestry Sciences,Bejing,China [3]Innovation Centerof Pesticide Research,Department of Applied Chemistry,College of Science,China Agricultural University,Bejing,China

出　　处：《Insect Science》2023年第3期611-624,共14页昆虫科学（英文版）

基　　金：China Agriculture Research System of MOF and MARA(CARS-24-C-03);National Key R&D Program of China(Grant no.2019YFD1002100).

摘　　要：Fatty acyl reductases(FARs)are key enzymes that participate in sex pheromone biosynthesis by reducing fatty acids to fatty alcohols.Lepidoptera typically harbor numerous FAR gene family members.Although FAR genes are involved in the biosynthesis of sex pheromones in moths,the key FAR gene of Spodoptera litura remains unclear.In this work,we predicted 30 FAR genes from the S.litura genome and identified a domain duplication within gene SlitFAR3,which exhibited high and preferential expression in the sexually mature female pheromone glands(PGs)and a rhythmic expression pattern during the scotophase of sex pheromone production.The molecular docking of SlitFAR3,as predicted using a 3D model,revealed a co-factor NADPH binding cavity and 2 substrate binding cavities.Functional expression in yeast cells combined with comprehensive gas chromatography indicated that the SlitFAR3 gene could produce fatty alcohol products.This study is the first to focus on the special phenomenon of FAR domain duplication,which will advance our understanding of biosynthesis-related genes from the perspective of evolutionary biology.

关 键 词：domain duplication fatty acyl reductase molecular docking SlitFAR3 Spodoptera litura yeast expression 

分 类 号：Q96[生物学—昆虫学]