Rho 1 participates in parasitoid wasp eggs maturation and host cellular immunity inhibition  

作　　者：Ruijuan Wang Zhe Lin Lizhen Zhou Caihua Chen Xianhao Yu Junje Zhang Zhen Zou Zhiqiang Lu 

机构地区：[1]Department of Entomology,College of Plant Protection,Northwest A&F University,Yangling,Shaanxi Province,China [2]State Key Laboratory of Integrated Management of Pest Insects and Rodents,Institute of Zoology,Chinese Academy of Sciences,Bejing,China [3]CAS Center for Excellence in Biotic Interactions,University of Chinese Academy of Sciences,Bejing,China [4]Engineering Research Center of Natural Enemies,Institute of Biological Control,Jilin Agricultural University,Changchun,Jilin,China

出　　处：《Insect Science》2023年第3期677-692,共16页昆虫科学（英文版）

基　　金：the National Natural Science Foundation of China(grants numbers 31772530,31970467 and 31872298);Strategic Priority Research Program of Chinese Academy of Sciences(Grant No.XDPB16).

摘　　要：Endoparasitoid wasps introduce venom into their host insects during the egglaying stage.Venom proteins play various roles in the host physiology,development,immunity,and behavior manipulation and regulation.In this study,we identified a venom protein,MmRhol,a small guanine nucleotide-binding protein derived from ovary in the endoparasitoid wasp Microplitis mediator and found that knockdown of its expression by RNA interference caused down-regulation of vitellogenin and juvenile hormone,egg production,and cocoons formation in the female wasps.We demonstrated that MmRho1 entered the cotton bollworm's(host)hemocytes and suppressed cellular immune responses after parasitism using immunofluorescence staining.Furthermore,wasp MmRhol interacted with the cotton bollworm's actin cytoskeleton rearrangement regulator diaphanous by yeast 2-hybrid and glutathione s-transferase pull-down.In conclusion,this study indicates that MmRho1 plays dual roles in wasp development and the suppression of the host insect cellular immune responses.

关 键 词：cellular immune responses diaphanous endoparasitoid wasp juvenile hormone small guanine nucleotide-binding protein VITELLOGENESIS 

分 类 号：Q96[生物学—昆虫学]