Characterization of neutral lipases revealed the tissue-specific triacylglyceroi hydrolytic activity in Nilaparvata lugens  

作　　者：Hui-Hui Zhang Bao-Jun Yang Yong Wu Hao-Li Gao Xu-Min Lin Jian-Zheng Zou Ze-Wen Liu 

机构地区：[1]Key Laboratory of Integrated Management of Crop Diseases and Pests(Ministry of Education),College of Plant Protection,Nanjing Agricultural University,Nanjing,China [2]Rice Technology Research and Development Center,China National Rice Research Institute,Hangzhou,China

出　　处：《Insect Science》2023年第3期693-704,共12页昆虫科学（英文版）

基　　金：the National Natural Science Foundation of China(grant number 31830075);the National Key Research and Development Program of China(2021YFD1401100).

摘　　要：The lipid metabolism plays an essential role in the development andreproduction of insects, and lipases are important enzymes in lipid metabolism. In Nilaparvatalugens, an important insect pest on rice, triacylglycerol hydrolytic activities weredifferent among tissues, with high activity in integument, ovary, and fat body, but low activityin intestine. To figure out the tissue-specific triacylglycerol hydrolytic activity, weidentified 43 lipases in N. lugens. Of these 43 lipases, 23 belonged to neutral lipases, sothis group was selected to perform further experiments on triacylglycerol hydrolysis. Thecomplete motifs of catalytic triads, β9 loop, and lid motif, are required for the triacylglycerolhydrolytic activity in neutral lipases, which were found in some neutral lipases withhigh gene expression levels in integument and ovary, but not in intestine. The recombinantproteins of 3 neutral lipases with or without 3 complete motifs were obtained, and the activitydetermination confirmed the importance of 3 motifs. Silencing XM_022331066.1,which is highly expressed in ovary and with 3 complete motifs, significantly decreasedthe egg production and hatchability of N. lugens, partially through decline of the lipidmetabolism. In summary, at least one-third of important motifs were incomplete in allneutral lipases with high gene expression in intestine, which could partially explain whythe lipase activity in intestine was much lower than that in other tissues. The low activityto hydrolyze triacylglycerol in N. lugens intestine might be associated with its food resourceand nutrient components, and the ovary-specific neutral lipases were important forN. lugens reproduction.

关 键 词：lipases neutral lipases Nilaparvata lugens REPRODUCTION triacylglycerol hydrolytic activity 

分 类 号：Q96[生物学—昆虫学]