Obstructor,a Frankliniella occidentalis protein,promotes transmission of tomato spotted wilt orthotospovirus  

作　　者：Xiaobin Zheng Yanran Wan Min Tao Jiangjiang Yuan Kun Zhang Jing Wang Youjun Zhang Pei Liang Qingjun Wu 

机构地区：[1]Department of Plant Protection,Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Bejing,China [2]Department of Entomology,China Agricultural University,Bejing,China

出　　处：《Insect Science》2023年第3期741-757,共17页昆虫科学（英文版）

基　　金：the China Agriculture Research System(CARS-24-C-02);the National Natural Science Foundation of China(32272548);the Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences(CAAS-ASTIP-IVFCAAS);the Hainan Major Science and Technology Project(ZDKJ2021007)。

摘　　要：Tomato spotted wilt orthotospovirus(TSWV)causes substantial economic losses to vegetables and other crops.TSWV is mainly transmitted by thrips in a persistent and proliferative manner,and its most efficient vector is the western flower thrips,Frankliniella occidentalis(Pergande).In moving from the thrips midgut to the salivary glands in preparation for transmission,the virions must overcome multiple barriers.Although several proteins that interact with TSWV in thrips have been characterized,we hypothesized that additional thrips proteins interact with TSWV and facilitate its transmission.In the current study,67 F occidentalis proteins that interact with GN(a structural glycoprotein)were identified using a split-ubiquitin membrane-based yeast 2-hybrid(MbY2H)system.Three proteins,apolipoprotein-D(ApoD),orai-2-like(Orai),and obstructor-E-like isoform X2(Obst),were selected for further study based on their high abundance and interaction strength;their interactions with Gn were confirmed by MbY2H,yeastβ-galactosidase and luciferase complementation assays.The relative expressions of ApoD and Orai were significantly down-regulated but that of Obst was significantly up-regulated in viruliferous thrips.When interfering with Obst in larval stage,the TSWV acquisition rate in 3 independent experiments was significantly decreased by 26%,40%,and 35%,respectively.In addition,when Obst was silenced in adults,the virus titer was significantly decreased,and the TSWV transmission rate decreased from 66.7%to 31.9%using the leaf disk method and from 86.67%to 43.33%using the living plant method.However,the TSWV acquisition and transmission rates were not affected by interference with the ApoD or Orai gene.The results indicate that Obst may play an important role in TSWV acquisition and transmission in Frankliniella occidentalis.

关 键 词：Frankliniella occidentalis obstructor tomato spotted wilt orthotospovirus TRANSMISSION virus-vector interaction 

分 类 号：S43[农业科学—农业昆虫与害虫防治]