Fumarate mitigates disruption induced by fenpropathrin in the silkworm Bombyx mori(Lepidoptera):A metabolomics study  

作　　者：Xue-Yang Wang Zi-Qin Zhao Cheng-Xian Song Zhi-Hao Su Mu-Wang Li Yang-Chun Wu Byung Rae Jin Ming-Jie Deng 

机构地区：[1]Jiangsu Key Laboratory of Sericultural Biology and Biotechnology,School of Biotechnology,Jiangsu University of Science and Technology,Zhenjang,Jiangsu Province,China [2]The Key Laboratory of Silkworm and Mulberry Genetic Improvement,Ministry of Agriculture,Sericultural Research Institute,Chinese Academy of Agricultural Science,Zhenjiang,Jiangsu Province,China [3]College of Natural Resources and Life Science,Dong-A University,Busan,Republic of Korea [4]Analytical and Testing Center,School of Pharmaceutical Sciences,Wenzhou Medical University,Wenzhou,Zhejiang Province,China

出　　处：《Insect Science》2023年第3期789-802,共14页昆虫科学（英文版）

基　　金：the National Natural Science Foundation of China(31802137);the Jiangsu Government Scholarship for Overseas Studies(JS-2019-230);the Zhejiang Provincial Natural Science Foundation(No.LY20C040002);the Postgraduate Research and Practice Innovation Program of Jiangsu Province(SJCX22_1993).

摘　　要：The silkworm Bombyx mori L.is a model organism of the order Lepidoptera.Understanding the mechanism of pesticide resistance in silkworms is valuable for Lepidopteran pest control.In this study,comparative metabolomics was used to analyze the metabolites of 2 silkworm strains with different pesticide resistance levels at 6,12,and 24 h after feeding with fenpropathrin.Twenty-six of 27 metabolites showed significant differences after fenpropathrin treatment and were classified into 6 metabolic pathways:glycerophospholipid metabolism,sulfur metabolism,glycolysis,amino acid metabolism,the urea cycle,and the tricarboxylic acid(TCA)cycle.After analyzing the percentage changes in the metabolic pathways at the 3 time points,sulfur metabolism,glycolysis,and the TCA cycle showed significant responses to fenpropathrin.Confirmatory experiments were performed by feeding silkworms with key metabolites of the 3 pathways.The combination of iron(II)fumarate+folic acid(IF-FA)enhanced fenpropathrin resistance in silkworms 6.38 fold,indicating that the TCA cycle is the core pathway associated with resistance.Furthermore,the disruption of several energy-related metabolic pathways caused by fenpropathrin was shown to be recovered by IF-FA in vitro.Therefore,IF-FA may have a role in boosting silkworm pesticide resistance by modulating the equilibrium between the TCA cycle and its related metabolic pathways.

关 键 词：Bombyx mori FENPROPATHRIN IF-FA metabolomics resistant mechanism 

分 类 号：O62[理学—有机化学]