青光安Ⅱ号方安全性及对慢性高眼压大鼠视神经的保护机制  

Study on the safety of Qingguang’anⅡFormula and its protective mechanism on the optic nerve of chronic hypertension rats

作　　者：蒋鹏飞[1] 曾志成[1] 刘冬华 刘培 颜春薇 谭诗 彭俊[2] 彭清华[1] JIANG Peng-fei;ZENG Zhi-cheng;LIU Dong-hua;LIU Pei;YAN Chun-wei;TAN Shi;PENG Jun;PENG Qing-hua(Hunan University of Chinese Medicine,Changsha 410208,China;First Affiliated Hospital ofHunan University of Chinese Medicine,Changsha 410007,China)

机构地区：[1]湖南中医药大学,长沙410208 [2]湖南中医药大学第一附属医院,长沙410007

出　　处：《中华中医药杂志》2023年第5期2010-2015,共6页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.81874492,No.82274588);湖南省研究生科研创新项目(No.CX20210695);湖南中医药大学中医学国内一流建设学科资助项目(No.2018-2022);湖南中医药大学中医学一流学科开放基金重点项目(No.2021ZYX13)。

摘　　要：目的:观察青光安Ⅱ号方的安全性及对慢性高眼压大鼠模型视神经的保护机制。方法:将50只SPF级SD大鼠按随机数表法分为空白组、模型组、益脉康组、青光安Ⅱ号方正常剂量组(正常剂量组)、青光安Ⅱ号方高剂量组(高剂量组),每组10只。除空白组与高剂量组外,其余大鼠的右眼采用灼烧巩膜静脉的方法建立慢性高眼压模型。空白组与模型组灌胃蒸馏水,益脉康组灌胃益脉康分散片混悬液,正常剂量组与高剂量组分别灌胃不同剂量青光安Ⅱ号方颗粒溶液。记录各组大鼠体质量与眼压,灌胃35 d取材,HE染色观察脏器与视网膜结构,Western Blot法检测大鼠视网膜白细胞介素(IL)-18、IL-1β、NOD样受体蛋白结构域相关蛋白3(NLRP3)、胱天蛋白酶(Caspase)-1、Caspase-8、瞬时受体电位香草酸4(TRPV4)蛋白的表达情况。结果:灌胃35 d后,空白组与高剂量组大鼠体质量无显著性差异;高剂量组大鼠各脏器与视网膜均未发现明显病理改变;各造模组大鼠造模后眼压从第1天开始,眼压均较造模前升高(P<0.05);模型组大鼠视网膜IL-18、IL-1β、NLRP3、Caspase-1、Caspase-8、TRPV4蛋白表达量较空白组显著增多(P<0.05),正常剂量组大鼠视网膜IL-18、IL-1β、NLRP3、Caspase-1、Caspase-8、TRPV4蛋白表达量较模型组显著减少(P<0.05)。结论:临床最大剂量青光安Ⅱ号方对大鼠重要脏器及视网膜无明显影响,青光安Ⅱ号方能下调慢性高眼压大鼠模型视网膜中TRPV4的表达量,进而抑制炎症因子与凋亡因子IL-18、IL-1β、NLRP3、Caspase-1、Caspase-8的表达,这可能是其保护视神经的主要机制之一。Objective:To observe the safety of Qingguan’anⅡFormula and its protective mechanism on the optic nevrve of rats with chronic ocular hypertension.Methods:Fifty SPF SD rats were randomly divided into blank group,model group,Yimaikang group,normal dose group of Qingguang’anⅡFormula(normal dose group)and high dose group of Qingguang’anⅡFormula(high dose group),with 10 rats in each group.In addition to the blank group and the high dose group,the right eyes of the rest rats were burned to establish the chronic ocular hypertension model.The blank group and the model group were perfused with distilled water,the Yimaikang group was perfused with Yimaikang dispersible tablets suspension,and the normal dose group and the high dose group were perfused with different doses of Qingguang’anⅡgranules solution.The weight and intraocular pressure of rats in each group were recorded.The samples were taken after 35 days of intragastric administration.The organs and retinal structures were observed by HE staining.The protein expressions of IL-18,IL-1β,NLRP3,Caspase-1,Caspase-8 and TRPV4 in the retina of rats were detected by Western Blot method.Results:After 35 days of intragastric administration,there was no significant difference in the body weight between the blank group and the high dose group;No obvious pathological changes were found in the organs and retinas of rats in the high dose group;From the first day after modeling,the intraocular pressure of rats in each model group was higher than that before modeling(P<0.05);The protein expressions of IL-8,IL-1β,NLRP3,Caspase-1,Caspase-8 and TRPV4 in retina of rats in model group were significantly higher than those in the blank group(P<0.05),and the protein expressions of IL-8,IL-1β,NLRP3,Caspase-1,Caspase-8 and TRPV4 in the normal dose group were significantly lower than those in the model group(P<0.05).Conclusion:The maximum clinical dose of Qingguan’anⅡFormula has no obvious effect on the important organs and retina of rats.Qingguan’anⅡFormula can down

关 键 词：青光安Ⅱ号方 青光眼 瞬时受体电位香草酸4 炎症因子 视神经 机制 安全性 

分 类 号：R285.5[医药卫生—中药学]