基于UPLC-MS/MS的肝肾亏虚型糖尿病远端对称性多发性神经病变患者代谢组学研究  

Metabolomics study of diabetic distal symmetric polyneuropathy with liver-kidney deficiency pattern based on UPLC-MS/MS

作　　者：许嘉慧 陈清光[1] 韩煦 田静 闫子惠 郭秋月 金燊懿 陆灏[1] XU Jia-hui;CHEN Qing-guang;HAN Xu;TIAN Jing;YAN Zi-hui;GUO Qiu-yue;JIN Shen-yi;LU Hao(Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China)

机构地区：[1]上海中医药大学附属曙光医院,上海201203

出　　处：《中华中医药杂志》2023年第5期2398-2402,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.82074381,No.82104786);上海市临床重点专科建设项目-中医内分泌(No.shslczdzk05401);华东片区及市级中医专科专病联盟建设-长三角中医内分泌代谢病专科联盟建设[No.ZY(2021-2023)-0302]。

摘　　要：目的:探索肝肾亏虚型糖尿病远端对称性多发性神经病变(DSPN)代谢物特征及潜在生物标志物。方法:采用超高效液相色谱-串联质谱(UPLC-MS/MS)方法对30例正常健康人群(Control组),30例2型糖尿病患者(T2DM组),30例肝肾亏虚型DSPN患者(LK DSPN组),30例非肝肾亏虚型DSPN患者(no-LK DSPN组)血浆代谢物进行检测,结合多变量统计分析中主成分分析、正交偏最小二承判别分析与单变量分析中显著性检验相结合的模式识别标志性差异代谢物,并对其代谢途径进行深入分析。结果:鉴定出γ-谷氨酰蛋氨酸和3-甲基戊烯二酸为LK DSPN组中标志性代谢物,较Control组、T2DM组和no-LK DSPN组,其含量在LK DSPN组中显著升高且表现出递增趋势。结论:γ-谷氨酰蛋氨酸和3-甲基戊烯二酸可能成为肝肾亏虚型DSPN中的生物标志物。Objective:To explore the metabolite characteristics and potential biomarkers of diabetic distal symmetric polyneuropathy(DSPN)with liver-kidney deficiency pattern.Methods:Plasma samples from 30 healthy people(Control group),30 type 2 diabetes mellitus(T2DM)patients(T2DM group),30 diabetic DSPN patients with liver-kidney deficiency pattern(LK group)and 30 diabetic DSPN patients without liver-kidney deficiency pattern(no-LK group)were measured by non-targeted metabonomics method of ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).The PCA and OPLS-DA analyses in multivariate statistical analysis and significance test in univariate analysis were performed to identify signature differential metabolites,and their metabolic pathways were analyzed.Results:Gamma-glutamylmethionine and 3-methylglutaconic acid were identified as signature metabolites in the LK DSPN group.Compared with the Control group,the T2DM group and the no-LK DSPN group,their contents were significantly increased in the LK DSPN group and showed an increasing trend.Conclusion:Gammaglutamylmethionine and 3-methylglutaconic acid might become the biomarkers of DSPN with liver-kidney deficiency pattern.

关 键 词：糖尿病远端对称性多发性神经病变 肝肾亏虚证 代谢组学 生物标志物 超高效液相色谱-串联质谱 

分 类 号：R587.2[医药卫生—内分泌] R745.44[医药卫生—内科学]