芪黄益肾方调控Lnc RNA MALAT1及Wnt/β-catenin通路抑制糖尿病视网膜病变大鼠上皮-间充质转化的作用  

Effects of Qihuang Yishen Formula in inhibiting epithelial-mesenchymal transition via regulating LncRNA MALAT1 and Wnt/β-catenin pathway in diabetic retinopathy rats

作　　者：袁丽莎 张宁[1] 刘世巍[1] 李同侠[1] 任秋月 杨荣禄 柳诗意[1] 石凯峰[1] 孟祥飞[1] YUAN Li-sha;ZHANG Ning;LIU Shi-wei;LI Tong-xia;REN Qiu-yue;YANG Rong-lu;LIU Shi-yi;SHI Kai-feng;MENG Xiang-fei(Department of Nephrology and Endocrinology,Wangjing Hospital of CACMS,Beijing 100102,China;Beijing University of Chinese Medicine,Beijing 100029,China)

机构地区：[1]中国中医科学院望京医院肾病内分泌科,北京100102 [2]北京中医药大学,北京100029

出　　处：《中华中医药杂志》2023年第5期2403-2408,共6页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.81973801)。

摘　　要：目的:观察芪黄益肾方(QHYS)调控LncRNA MALAT1及Wnt/β-catenin通路对糖尿病视网膜病变(DR)大鼠视网膜组织上皮-间充质转化(EMT)的作用。方法:高脂高糖饮食联合链脲佐菌素腹腔注射构建DR大鼠模型,QHYS干预24周。检测血糖、血脂;视网膜组织病理染色;Western Blot检测视网膜组织EMT标志物和Wnt/β-catenin通路蛋白表达;RT-qPCR法检测LncRNA MALAT1表达。结果:与模型组比较,QHYS中剂量组空腹血糖、甘油三脂显著降低(P<0.01,P<0.05),平均视网膜厚度增加(P<0.05),E-cad、RPE65表达上调(P<0.01),N-cad、α-SMA表达下调(P<0.05,P<0.01),Wnt1、β-catenin、active-β-catenin、LncRNA MALAT1表达均下调(P<0.01,P<0.05)。QHYS低、高剂量组的上述结果有不同程度改善。结论:QHYS可降低DR大鼠血糖、血脂,改善视网膜组织形态,抑制EMT,可能是通过下调LncRNAMALAT1表达及抑制Wnt/β-catenin通路的激活实现。Objective:To observe the effects of Qihuang Yishen Formula(QHYS)on the epithelial-mesenchymal transformation(EMT)of retinal tissue in diabetic retinopathy(DR)rats by regulating LncRNA MALAT1 and Wnt/β-catenin pathway.Methods:The DR rat model was established by the method of high-fat and high-sugar diet combined with intraperitoneal injection of streptozotocin.The intervention of QHYS lasted for 24 weeks.Fasting plasma glucose and triglyceride were detected;HE staining was performed on retinal tissues.The expressions of EMT markers and Wnt/β-catenin pathway proteins in retinal tissues were detected by Western Blot.The expression of LncRNA MALAT1 was detected by RT-qPCR.Results:Compared with model group,fasting plasma glucose and triglyceride were significantly decreased(P<0.01,P<0.05),and the average retinal thickness was increased(P<0.05),and the expressions of E-cad and RPE65 were significantly up-regulated(P<0.01),and the expressions of N-cad andα-SMA were significantly down-regulated(P<0.05,P<0.01),and the expressions of Wnt1,β-catenin,active-β-catenin and LncRNA were significantly down-regulated(P<0.01,P<0.05)in QHYS medium-dose group.The above results were improved to different degrees in QHYS low dose and high dose groups.Conclusion:QHYS can reduce fasting plasma glucose and triglyceride,improve retinal morphology and inhibit EMT in DR rats,which may be achieved by down-regulating LncRNA MALAT1 expression and inhibiting the activation of Wnt/β-catenin pathway.

关 键 词：芪黄益肾方 中药 糖尿病视网膜病变 糖尿病微血管并发症 上皮-间充质转化 长链非编码RNA WNT/Β-CATENIN 

分 类 号：R285.5[医药卫生—中药学]