Protective effect of Tongdu Tiaoshen acupuncture combined with Xiaoxuming decoction(小续命汤)on dopaminergic neurons in Parkinson’s disease model  

作　　者：WU Haiyang WANG Ying HAN Wei LI Huihui JI Haisheng LIU Xiuxiu 

机构地区：[1]Department of encephalopathy,the Second Affiliated Hospital of Anhui University of Traditional Chinese Medicine,Hefei 230061,China [2]Deparment of acupuncture and Moxibustion,The Affiliated Brain Hospital of Guangzhou Medical University,Guangzhou 510370,China [3]Department of acupuncture and massage,Anhui University of Chinese Medicine,Hefei 230061,China

出　　处：《Journal of Traditional Chinese Medicine》2023年第3期484-493,共10页中医杂志（英文版）

基　　金：Supported by National Natural Science Foundation of China:Study on the Mechanism of Regulating miR-124 by Tongdu Tiaoshen Acupuncture to Promote Neuroprotection in Cerebral Ischemia Reperfusion Injury(No.81973933);Natural Science Fund for Colleges and Universities in Anhui Province:Study on the Regulatory Mechanism of Tongdu Tiaoshen Acupuncture on Dopaminergic Neurons in Parkinson’s Disease Model Mice Based on Mitochondrial Autophagy(No.KJ2019A0475)。

摘　　要：OBJECTIVE:To explore the possible mechanism of Tongdu Tiaoshen acupuncture combined with Xiaoxuming decoction(小续命汤,XXMD)in the treatment of Parkinson’s disease(PD).METHODS:C57BL/6 mice were randomly divided into eight groups(n=12),including blank group,model group,medication group,acupuncture group,high-dose XXMD group(XXMD-H),low-dose XXMD group(XXMD-L),acupuncture combined with high-dose XXMD group(A+H),and acupuncture combined with low-dose XXMD group(A+L).After treatment for 6 weeks,dopamine(DA)neurons and the pathological changes of tyrosine hydroxylase(TH)positive cells were observed.The enzyme-linked immunosorbent assay(ELISA)was used to measure the content of DA and the level of interleukin-1β(IL-1β),interleukin-6(IL-6),interleukin-10(IL-10)and tumor necrosis factor alpha(TNF-α).The m RNA level of PINK1 and Parkin and the protein expression of Nix,PINK1 and Parkin in the substantia nigra were also detected.RESULTS:Combination treatment effectively ameliorated the symptoms of PD.Compared with model group,combined treatment significantly up-regulated the protein expression of Nix,Parkin and PINK1 and the m RNA levels of PINK1 and Parkin in the substantia nigra(P<0.0001,P<0.001,P<0.01 or P<0.05).Furthermore,the levels of pro-inflammation cytokines were obviously decreased after combination therapy,while IL-10 content was increased remarkably(P<0.01).CONCLUSION:Compared with each treatment alone,combination therapy improved the pathological damage of DA neurons of PD mice more effectively.The possible mechanism may be attributed to the up-regulated level of mitochondrial autophagy and improved mitochondrial function.These results provide fresh insight into the mechanism of co-treatment with Tongdu Tiaoshen acupuncture and XXMD for PD.

关 键 词：Parkinson disease ACUPUNCTURE dopaminergic neurons Tongdu Tiaoshen Xiaoxuming decoction mitochondrial autophagy 

分 类 号：R285.5[医药卫生—中药学]