Waggle needling Yanglingquan(GB34) enhances cerebral blood flow and ameliorates condition of spastic paresis in rats with middle cerebral artery occlusion induced by surgical nylon suture  

作　　者：ZHANG Zhou MU Jiedan MA Liangxiao YU Wenyan SUN Tianyi QIAN Xu TIAN Yuan ZHANG Yidan WANG Junxiang 

机构地区：[1]School of Acupuncture-Moxibustion and Tuina,Beijing University of Chinese Medicine,Beijing 100029,China [2]the Key Unit of State Administration of Traditional Chines Medicine,Evaluation of Characteristic Acupuncture Therapy,Beijing 100029,China [3]School of Nursing,Beijing University of Chinese Medicine,Beijing 100029,China

出　　处：《Journal of Traditional Chinese Medicine》2023年第3期514-522,共9页中医杂志（英文版）

基　　金：Supported by Scientific Research Development Fund Program of National Natural Science Foundation of China:Study on the Mechanism of Waggle Needling GB34 Relieving Spasticity in Poststroke Rats Based on KCC2-GABAA Receptor Pathway(No.81774417);the Fundamental Research Funds for the Central Universities of China:Research and Development of Physical Therapy Technology and Equipment for Regulation of Human Functional State(No.2020-JYB-ZDGG-062)。

摘　　要：OBJECTIVE:To evaluate the therapeutic efficacy and the influence on cerebral blood supply of waggle needling Yanglingquan(GB34) on spastic paresis(SP) rats after middle cerebral artery occlusion(MCAO) induced and investigate its mechanism of relieving neurobehavior deficiency.METHODS:SP rat model was produced by permanent MCAO.Rats were divided into five groups:blank control group(Control),sham operation group(Sham),model group(Model),waggle needling group(WN) and perpendicular needling group(PN).SP rats were treated with acupuncture from day 3 after MCAO,once a day for 6 d.The modified neurological severity score(m NSS) and modified Ashworth scale(MAS) were conducted on days 0,1,3,5,7 and 9.Cerebral blood flow(CBF) in ischemic cortex was measured by laser speckle imaging 5 min pre ischemia,5 min post ischemia,and after intervention on day 9.All rats were sacrificed at day 9 and the protein and m RNA expressions of γ2 subunit of the γ-aminobutyric acid receptor A(GABAAγ2) and K+-Cl-cotransporter 2(KCC2) in the ischemic cortex and lumber enlargement was measured by Western blotting and real-time quantitative polymerase chain reaction.RESULTS:Both Control and Sham groups showed no changes in m NSS and MAS scores and in the regional CBF.Compared with Model group,both WN and PN treatments significantly ameliorated neurological deficit(P < 0.01),decreased muscle tone(P < 0.05),and enhanced CBF(P < 0.001) in SP rats;moreover,WN showed superior effects than PN(P < 0.001).In line with the improvement in neurobehavior,acupuncture interventions up-regulated the expressions of GABAAγ2 and KCC2 in the ischemic cortex as well as lumber enlargement(P < 0.01) in SP rats,and those changes were more obvious in WN(P < 0.05).CONCLUSIONS:Acupuncture at Yanglingquan(GB34) enhanced cerebral blood flow and ameliorated SP in permanent MCAO rats,while waggle needling was superior to regular perpendicular needling.Waggle needling Yanglingquan(GB34) would be a potential complementary therapy for SP.

关 键 词：spastic paresis acupuncture cerebrovascular circulation laser speckle imaging γ2 subunit of theγ-aminobutyric acid receptor A K+-Cl-cotransporter 2 

分 类 号：R245[医药卫生—针灸推拿学]