Efficacy of needling Baihui(GV20),Neiguan(PC6),Shenmen(HT7) and Taichong(LR3) on cerebral cortical blood oxygen level in rats with insomnia  

作　　者：YE Yujuan WEI Yuting JIA Jing YAN Xingke 

机构地区：[1]College of Acupuncture and Massage,Gansu University of Chinese Traditional Medicine,Lanzhou 730000,China

出　　处：《Journal of Traditional Chinese Medicine》2023年第3期523-532,共10页中医杂志（英文版）

基　　金：Supported by Open Project of Gansu Research Center of Traditional Chinese Medicine in 2020-study on the Regulatory Effect and Brain Mechanism of Acupuncture on Insomnia based on “Neurovascular Coupling”(zyzx-2020-zx13);Study on the Intervention Effect and Brain Mechanism of Acupuncture on PTSD Induced by Catastrophic Stress(21JR1RA266);Study on the Effect of Acupuncture on the Expression of Hippocampal 5-HT1AR, 5-HT2AR and Hypothalamic GABAAR-α1 in Sleep Deprived Rats(ZJKF2006)。

摘　　要：OBJECTIVE:To evaluate the effect of needling Baihui(GV20),Neiguan(PC6),Shenmen(HT7) and Taichong(LR3) on cerebral cortical blood oxygen level in rats with insomnia identified as liver-Qi stagnation pattern in terms of Traditional Chinese Medicine.METHODS:Sixty Wistar rats were randomly divided into blank group(n = 10),the rest of the rats were clamped tail as stimulation combined with injected intraperitoneally p-chlorophenylalanine(PCPA) to establish sleep deprivation model.After the successful replication of the model,they were randomly divided into model group,grabbing group,Western Medicine group,acupuncture group and sham acupuncture group,with 10 rats in each group.The model group was given normal saline;The grasping group was grasped in the same way as the two treatment groups;Western medicine group was given estazolam solution;The acupuncture group was treated with the acupuncture method of "soothing the liver Qi and regulating mind" needling Baihui(GV20),Neiguan(PC6),Shenmen(HT7) and Taichong(LR3);The sham acupuncture group were needled with four nonacupoint sites.After 7 d of treatment of rats in each group,sodium pentobarbital sleep experiment was used to evaluate the sleep latency(SL) and sleep time(ST).The percentage of rats entering the open arm(OE%) and the percentage of time entering the open arm(OT%) in each group were monitored by elevated cross maze,and the vertical score,horizontal crossing times,central grid score and modification times were monitored by open field test,functional near infrared spectroscopy(f NIRs) was used to monitor the changes of oxygenated hemoglobin(Oxy-Hb),deoxyhemoglobin(Deoxy-Hb) and total hemoglobin(Total-Hb) in the cerebral cortex of rats in each group under light stimulation and dark stimulation,the statistically significant channel combinations were selected from 8 light sources and 12 detectors(S-D).According to the position of the light source detector covering the cerebral cortex,Preliminarily determine the key brain areas of insomnia(the preliminary experiment

关 键 词：sleep initiation and maintenance disorders acupuncture spectroscopy near-infrared HEMOGLOBINS brain function 

分 类 号：R245[医药卫生—针灸推拿学]