In vitro assessment of antioxidant,neuroprotective,anti-urease and anti-tyrosinase capacities of Tamarix africana leaves extracts  

作　　者：Esma Anissa Trad Khodja Abd El Hamid Khabtane Rabah Arhab Djamila Benouchenne Mohamed Sabri Bensaad Chawki Bensouici Ramazan Erenler 

机构地区：[1]Biotechnology,Water,Environment and Health Laboratory,Faculty of Natural and Life Sciences,University Abbes Laghrour,Khenchela 40000,Algeria [2]Laboratory of Natural Substances,Biomolecules and Biotechnological Applications,Faculty of Natural and Life Sciences,University Larbi Ben M Hidi,Oum El-Bouaghi 04000,Algeria [3]Genetics,Biochemistry and Plant Biotechnology Laboratory,Faculty of Natural and Life Sciences,University Constantine 1,Constantine 25000,Algeria [4]Pharmaceutical Sciences Research Center,Constantine 25000,Algeria [5]Laboratory of Cellular and Molecular Physio-Toxicology-Pathology and Biomolecules(LPTPCMB),Faculty of Natural and Life Sciences,University Batna 2,Batna 05000,Algeria [6]Laboratory of Biotechnology of Bioactive Molecules and Cellular Physiopathology(LBMBPC),Faculty of Natural and Life Sciences University Batna 2,Batna 05000,Algeria [7]Biotechnology Research Center Ali Mendjli UV 03,Constantine 25000,Algeria [8]Department of Chemistry,Faculty of Science and Literature,University TokatGaziosmanpasa,Tokat 60250,Turkey

出　　处：《Journal of Traditional Chinese Medicine》2023年第2期252-264,共13页中医杂志（英文版）

摘　　要：OBJECTIVE:To characterize the chemical profile of methanolic crude extract and its fractions(Ethyl acetate,n-butanol and aqueous)using liquid chromatographymass spectrometry(LC-MS)analysis,to evaluate their biological and pharmacological properties:antioxidant(1,1-diphenyl-2-pycrylhydrazyl(DPPH),2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic)(ABTS·+),galvinoxyle free radical scavenging,reducing power,phenanthroline andβcarotene-linoleic acid bleaching assays),enzymes inhibitory ability against several enzymes[acetylcholinesterase(AChE),buthyrylcholinesterase(BChE),urease and tyrosinase].METHODS:Secondary metabolites were extracted from Tamarix africana air-dried powdered leaves by maceration,the crude extract was fractionated using different solvents with different polarities(Ethyl acetate,n-butanol and aqueous).The amount of polyphenols,flavonoids and tannins(hydrolysable and condensed)were determined using colorimetric assays.A variety of in vitro biochemical tests were carried out to assess antioxidant and oxygen radical scavenging properties using DPPH,ABTS,galvinoxyle free radical scavenging,reducing power,phenanthroline andβcarotene-linoleic acid bleaching methods.Neuroprotective effect was examined against acetylcholinesterase and buthyrylcholinesterase enzymes.The anti-urease and antityrosinase activities were performed against urease and tyrosinase enzymes respectively.The extract’s components were identified using LC-MS and compared to reference substances.RESULTS:The results indicated that Tamarix africana extracts presented a powerful antioxidant activity in all assays and exhibited a potent inhibitory effect against AChE and BChE as well as urease and tyrosinase enzymes.LC-MS analysis identified amount of eight phenolic compounds were revealed in this analysis;Apigenin,Diosmin,Quercetin,Quercetine-3-glycoside,Apigenin 7-O glycoside,Rutin,Neohesperidin and Wogonin in methanolic extract and its different fractions of Tamarix africana from leaves.CONCLUSIONS:Based on these findings,it is reaso

关 键 词：ANTIOXIDANTS anti-tyrosinase anti-urease liquid chromatography-mass spectrometry neuroprotection phenolic content Tamarix africana 

分 类 号：R285[医药卫生—中药学]