Scalp acupuncture Yikang therapy on Baihui(GV20),Sishencong(EX-HN1),Zhisanzhen,Niesanzhen improves neurobehavior in young rats with cerebral palsy through Notch signaling pathway  

作　　者：XUE Jianyi XU Jinyan HUANG Mao YU Wentao YAN Yihui YAN Yuanjie YIN Zhenjin LU Qian PENG Wanying YAN Siyang 

机构地区：[1]Department of Acupuncture and Message,Hebei University of Chinese Medicine,Shijiazhuang 050091,China [2]Department of Child Rehabilitation,the First Affiliated Hospital of Hebei University of Chinese Medicine,Shijiazhuang 050013,China [3]the second Outpatient Department,Hebei University of Chinese Medicine,Shijiazhuang 050090,China [4]School of Basic Medicine,Hebei University of Chinese Medicine,Shijiazhuang 050200,China

出　　处：《Journal of Traditional Chinese Medicine》2023年第2期337-342,共6页中医杂志（英文版）

基　　金：Supported by Key Projects of Scientific Research in Higher Education Institutions in Hebei Province:Study on the Mechanism of Scalp Acupuncture Yikang Therapy in Cerebral Palsy Rats Based on Notch Signal Pathway(ZD2020144);Doctoral Research Project of Hebei University of Chinese Medicine:Study on the Mechanism of Scalp Acupuncture Yikang Therapy in Cerebral Palsy Rats Based on Notch Signal Pathway(BSZ2020002)。

摘　　要：OBJECTIVE:To investigate the efficacy of scalp acupuncture Yikang therapy on Baihui(GV20),Sishencong(EX-HN1),Zhisanzhen,Niesanzhen,on neurobehavior in young rats with cerebral palsy based on Notch signaling pathway.METHODS:Thirty 7-day-old rats were randomly divided into sham,model and acupuncture,10 rats in each group.The cerebral palsy model was established by the accepted modeling method,the acupuncture group selected"Baihui(GV20)","Sishencong(EX-HN1)","Zhisanzhen"and"Niesanzhen"for intervention 24 h after the model was made.The body masses were recorded before and after the treatment,respectively.After the intervention,the rats were subjected to suspension experiment,slope experiment,tactile stimulation experiment and Morris water maze experiment.After the end of the experiment,the morphological changes of hippocampal histology were observed by hematoxylineosin(HE)staining under light microscope,and the expression of Notch1,Notch3 and Hes5 were detected by Western blot and quantitative real-time polymerase chain reaction(PCR).RESULTS:The changes in body mass of the rats in each group were different;in behavioral experiments,compared with the sham,the suspension time of the model was shortened,the slope experiment,tactile stimulation experiment,and escape latency time were prolonged,and the number of platform crossing was reduced in the model,compared with the model,the suspension time of the acupuncture was prolonged,the slope experiment,tactile stimulation experiment,and escape latency time were shortened,and the number of platform crossing times was increased;HE staining showed severe hippocampal damage in the model and reduced hippocampal damage in the acupuncture.Western Blot and real-time fluorescence quantitative PCR showed that the expression of Notch1,Notch3 and Hes5 were increased in the model and the expression of Notch1,Notch3,Hes5 in acupuncture were decreased.CONCLUSIONS:Scalp acupuncture Yikang therapy may improve neurobehavior and reduce brain injury in rats with cerebral palsy by downregulatin

关 键 词：cerebral palsy scalp acupuncture Yikang therapy hippocampal tissue Notch signaling pathway 

分 类 号：R245[医药卫生—针灸推拿学]