电针“内关”预处理对心肌缺血再灌注损伤大鼠心肌Akt/mTOR通路的影响  

Effects of electroacupuncture preconditioning of"Neiguan"(PC 6)on myocardial ischemia reperfusion injury via Akt/mTOR pathway

作　　者：柳冬冬 何妙嬴 张燚格 李丹[1] 张程程[1] 王超[1] Dongdong Liu;Miaoying He;Yige Zhang;Dan Li;Chengcheng Zhang;Chao Wang(College of Acupuncture&Tuina and Rehabilitation,Hunan University of Chinese Medicine,Changsha 410006,China)

机构地区：[1]湖南中医药大学针灸推拿与康复学院,长沙410006

出　　处：《国际中医中药杂志》2023年第6期703-708,共6页International Journal of Traditional Chinese Medicine

基　　金：湖南省自然科学基金项目(2021JJ30507)。

摘　　要：目的观察电针预处理对心肌缺血再灌注损伤(MIRI)模型大鼠心肌组织自噬相关通路Akt/mTOR的影响,探讨电针“内关”对心肌损伤的作用机制。方法将48只SD大鼠按随机数字表法分为空白组、假手术组、模型组和内关组,每组12只。内关组电针刺激大鼠“内关”,每次30 min,1次/d,连续干预7 d。干预结束后,除空白组外,其余各组大鼠采用冠状动脉左前降支结扎法建立MIRI模型。采用HE染色法观察大鼠心肌组织病理形态学改变;Western blot法检测心肌组织Akt、磷酸化Akt(p-Akt)、mTOR、磷酸化mTOR(p-mTOR)蛋白表达,计算p-Akt/Akt、p-mTOR/mTOR比值。结果空白组心肌纤维排列规则整齐,心肌间质未见炎性细胞浸润、出血;假手术组心肌纤维排列稍不规则,未见断裂,可见少量心肌纤维间隙稍扩大;模型组心肌纤维分布紊乱,肥大心肌细胞增多,部分线粒体红肿或外膜破裂,间质可见炎性浸润、出血;内关组心肌组织病变程度较模型组减轻,少量间质出血及炎性细胞浸润。各组大鼠心肌组织Akt、mTOR蛋白表达比较差异无统计学意义(P>0.05);与假手术组比较,模型组p-Akt、p-mTOR表达及p-Akt/Akt、p-mTOR/mTOR降低(P<0.01);与模型组比较,内关组p-Akt、p-mTOR表达及p-Akt/Akt、p-mTOR/mTOR升高(P<0.01)。结论电针“内关”预处理可能通过激活MIRI大鼠心肌细胞Akt/mTOR通路抑制过度自噬,从而减轻MIRI,起到保护心肌的作用。Objective To observe the effects of electroacupuncture preconditioning on the autophagy-related pathway protein kinase B(Akt)/mammalian target of rapamycin(mTOR)in myocardial tissue of rats with myocardial ischemia reperfusion injury(MIRI);To investigate the protective mechanism of"Neiguan"(PC 6)on myocardial injury.Methods Totally 48 SD rats were randomly divided into blank group,sham-operation group,model group and Neiguan group(n=12 in each group).The Neiguan group was applied to bilateral"Neiguan"(PC 6)by electroacupuncture for 30 min,once daily for consecutive 7 days before model replication.Except in the blank group,the MIRI model was established by ligation of the descending anterior branch of the left coronary artery in the rest groups after the intervention.The histomorphological changes in the myocardium of the rats were observed by HE staining,and the expression levels of Akt,phosphorylated Akt(p-Akt),mTOR and phosphorylated mTOR(p-mTOR)in the myocardium were measured by protein immunoblotting.The ratio of p-Akt/Akt and p-mTOR/mTOR was calculated.Results In the blank group,the myocardial fibres were arranged regularly and neatly,and no inflammatory cell infiltration or haemorrhage was seen in the interstitium;in the sham-operation group,the arrangement of myocardial fibers was slightly irregular,no rupture was found,and a small amount of myocardial fiber gap was slightly enlarged;in the model group,the distribution of myocardial fibers was disordered,hypertrophic cardiomyocytes increased,some mitochondria were red and swollen or the outer membrane was ruptured,and inflammatory infiltration and hemorrhage were seen in the interstitium;the extent of myocardial lesions in the Neiguan group was less than that in the model group,with a small amount of interstitial hemorrhage and inflammatory cell infiltration.There was no statistical significance in the levels of Akt and mTOR in the myocardial tissues of the rats in each group(P>0.05);compared with the sham-operation group,the levels of p-Akt,p-mTOR and p-A

关 键 词：心肌再灌注损伤 自噬性细胞死亡 电针 预处理 穴 内关 大鼠 

分 类 号：R245[医药卫生—针灸推拿学]