基于UPLC指纹图谱及主成分分析法评价不同地区柴胡药材质量  

Qualitative evaluation of Bupleuri Radix in different areas by UPLC fingerprint combined with Principle Component Analysis

作　　者：魏娟 赵利军 张晓燕 祝宇 Juan Wei;Lijun Zhao;Xiaoyan Zhang;Yu Zhu(Department of Pharmacy,Shiyan Renmin Hospital,Renmin Hospital Affiliated to Hubei University of Medicine,Shiyan 442000,China;Hubei Key Laboratory of Wudang Local Chinese Medicine Research(Hubei University of Medicine),Shiyan 442000,China;BingBing HongKang Chinese Harbal Pieces(ShiYan)Co.,Ltd.,Shiyan 442510,China)

机构地区：[1]十堰市人民医院(湖北医药学院附属人民医院)药学部,十堰442000 [2]武当特色中药研究湖北省重点实验室,十堰442000 [3]兵兵宏康中药饮片(十堰)有限公司,十堰442510

出　　处：《国际中医中药杂志》2023年第6期725-729,共5页International Journal of Traditional Chinese Medicine

基　　金：湖北省教育厅自然科学基金项目(B2020110);2018年十堰市科学技术研究与开发项目(18Y72)。

摘　　要：目的建立柴胡药材的超高效液相色谱(UPLC)指纹图谱,结合主成分分析法评价不同地区柴胡药材质量。方法采用UPLC法,色谱柱为Acquity BEH-C18(2.1 mm×100 mm,1.7μm),流动相为乙腈(A)-水(B),梯度洗脱;柱温为30℃,流速为0.3 ml/min,检测波长为200 nm,进样量为5μl。结果10批柴胡药材的UPLC指纹图谱有7个共有峰,相似度为0.940~0.975。经主成分分析,3个主成分因子的累计方差贡献率为90.977%,以S5(湖北)药材样品的主成分因子综合得分最高,整体质量最好。结论不同地区柴胡药材存在一定质量差异,通过指纹图谱与主成分分析相结合,可为柴胡药材的质量控制及开发应用提供参考依据。Objective To establish the fingerprint of Bupleuri Radix with Ultra High Performance Liquid Chromatography(UPLC)method and combining Principal Component Analysis to evaluate the quality of Bupleuri Radix in different areas.Methods Acquity UPLC BEH-C18(2.1 mm×100 mm,1.7μm)column was used with acetonitrile(A)-water(B)solution,gradient elution.The column temperature was 30℃,the flow rate was 0.3 ml/min,and the detection wavelength was 200 nm,injection volume 5μl.Results There were 7 common peaks in the UPLC fingerprints of 10 batches of medicinal materials,and the similarity was 0.940-0.975.Through the principal component analysis,the cumulative contribution rate of three main component factors was 90.977%,and comprehensive score of S5(Hubei)was the highest with the best quality.Conclusions There are certain quality differences of different areas in Bupleuri Radix.Through the combination of fingerprint and principal component analysis,it can provide reference for quality control,development and application of Bupleuri Radix.

关 键 词：柴胡 指纹图谱 主成分分析 超高效液相色谱 中药产地 

分 类 号：R284.1[医药卫生—中药学]