基于网络药理学探讨三七抗肿瘤转移作用机制  

Discussion on the anti-tumor metastasis mechanism of Notoginseng Radix et Rhizoma based on internet pharmacology

作　　者：刘松江[1] 赵欣华 张东旭 隋博文[3] 李雨[1] 张俐佳 刘宇 庞雪莹 Songjiang Liu;Xinhua Zhao;Dongxu Zhang;Bowen Sui;Yu Li;Lijia Zhang;Yu Liu;Xueying Pang(Department of Oncology,the First Affiliated Hospital of Heilongjiang University of Chinese Medicine,Harbin 150040,China;Graduate School of Heilongjiang University of Chinese Medicine,Harbin 150036,China;Department of Respiratory Medicine,the First Affiliated Hospital of Heilongjiang University of Chinese Medicine,Harbin 150040,China;Base Office of the First Affiliated Hospital of Heilongjiang University of Chinese Medicine,Harbin 150040,China)

机构地区：[1]黑龙江中医药大学附属第一医院肿瘤科,哈尔滨150040 [2]黑龙江中医药大学研究生院,哈尔滨150036 [3]黑龙江中医药大学附属第一医院呼吸科,哈尔滨150040 [4]黑龙江中医药大学附属第一医院基地办,哈尔滨150040

出　　处：《国际中医中药杂志》2023年第6期749-754,共6页International Journal of Traditional Chinese Medicine

基　　金：黑龙江省应用技术研究与开发计划项目(GY2019YF0210)。

摘　　要：目的利用网络药理学方法探讨三七抗肿瘤转移的作用机制。方法检索TCMSP筛选三七的有效成分及靶点,运用GeneCards数据库筛选抗肿瘤转移相关靶点,将有效成分靶点与疾病靶点进行Venn分析,得到三七抗肿瘤转移的关键靶点。借助Cytoscape 3.7.2软件构建药物-成分-靶点-疾病网络。运用STRING数据库构建PPI网络。借助Bioconductor对靶点基因进行KEGG信号通路和GO生物过程富集分析。结果从三七中筛选出活性成分119个,符合条件的有效成分8个,对应162个相关靶点,与抗肿瘤转移有关靶点121个,PPI网络筛选出关键靶点30个,包括AKT1、MAPK1、JUN、RELA、IL6等。GO功能富集分析主要涉及细胞因子受体结合、血红素结合、RNA聚合酶Ⅱ转录因子结合、泛素蛋白连接酶结合、类固醇激素受体活性等生物过程,KEGG通路富集分析得到与三七抗肿瘤转移相关的信号通路149条,主要涉及AGE-RAGE、PI3K-Akt等信号通路,以及乙型肝炎、卡波西肉瘤相关疱疹病毒感染、人巨细胞病毒感染等多种病毒感染及多种肿瘤。结论三七可通过人参皂苷f2、人参皂苷rh2、β-谷甾醇、豆甾醇、槲皮素等多个活性成分,通过AKT1、MAPK1、JUN、RELA、IL6等多个靶点作用于PI3K-Akt等信号通路,发挥抗肿瘤转移作用。Objective To analyze and explore the possible mechanism of anti-tumor metastasis of Notoginseng Radix et Rhizoma using Internet pharmacology.Methods The active components and targets of Notoginseng Radix et Rhizoma were screened by retrieving Chinese Medicine System Pharmacology Database and Analysis Platform(TCMSP).GeneCards database was used to screen the anti-tumor metastasis-related targets,and compounds and disease targets were under mapping analysis.Key targets of Notoginseng Radix et Rhizoma for anti-tumor metastasis were screened through Venn map.With the help of Cytoscape 3.7.2 software,a compound-disease network diagram was constructed.String platform was used to build a PPI network.Bioconductor was used to enrich the target genes for KEGG signaling pathway and GO biological process analysis.Results Totally 119 active components were selected from Notoginseng Radix et Rhizoma.There were 8 eligible active components,corresponding to 162 related targets,121 targets related to anti-tumor metastasis,and 30 key targets screened by PPI network,including AKT1,MAPK1,JUN,RELA,IL6,etc.GO enrichment analysis mainly involved biological processes such as cytokine receptor binding,heme binding,RNA polymeraseⅡtranscription factor binding,ubiquitin protein ligase binding,and steroid hormone receptor activity.149 signal pathways related to Notoginseng Radix et Rhizoma anti-tumor metastasis were obtained by KEGG enrichment analysis,mainly involving multiple signal pathways,such as AGE-RAGE and PI3K-Akt,and hepatitis B,Kaposi's sarcoma-associated herpes virus infection,human cytomegalovirus infection and other viral infections and various tumors.Conclusion Notoginseng Radix et Rhizoma can pass multiple active components,such as ginsenoside f2,ginsenoside rh2β-,sitosterol,stigmasterol and quercetin,and multiple targets,such as AKT1,MAPK1,JUN,RELA and IL6,acting on multiple pathways such as PI3K-Akt,thereby playing the role of anti-tumor metastasis.

关 键 词：三七 网络药理学 抗肿瘤转移 药理作用分子作用机制(中药) 

分 类 号：R285[医药卫生—中药学]