姜黄素对高糖诱导人肾小管上皮细胞线粒体自噬的影响  

Effects of curcumin on mitochondrial autophagy induced by high glucose in human renal tubular epithelial cells

作　　者：常江[1] 王颖[2] 李永妍 赖连英 杨时旭 李玉洁 CHANG Jiang;WANG Ying;LI Yongyan;LAI Lianying;YANG Shixu;LI Yujie(Hepatobiliary Surgery Department,The First Affiliated Hospital of Hainan Medical College,HaiKou 570102,China;General Practice Department,the First Affiliated Hospital of Hainan Medical College;Hainan Medical College)

机构地区：[1]海南医学院第一附属医院肝胆外科,海南海口570102 [2]海南医学院第一附属医院全科医学科 [3]海南医学院

出　　处：《包头医学院学报》2023年第6期24-29,共6页Journal of Baotou Medical College

基　　金：海南省卫生健康行业科研项目(21A200073);海南省自然科学基金高层次人才项目(822RC826);海南医学院大学生创新创业训练计划项目(X202111810020)。

摘　　要：目的:观察姜黄素(Curcumin,Cur)对高糖环境下人肾小管上皮细胞(Human kidney 2,HK-2)线粒体自噬及氧化应激的影响,探究姜黄素对HK-2细胞的保护作用及机制。方法:体外培养HK-2细胞,随机分为4组:对照组(5.5 mmol/L葡萄糖)、姜黄素组(5.5 mmol/L葡萄糖+10μmol/L姜黄素)、高糖组(30 mmol/L葡萄糖)、姜黄素高糖组(30 mmol/L葡萄糖+10μmol/L姜黄素)。各组干预刺激48 h,收集细胞的总蛋白,采用Western blot检测线粒体自噬相关蛋白Pink1、Parkin、LC3B以及beclin1的表达;JC-1检测各组线粒体膜电位水平;采用活性氧检测试剂盒检测细胞内活性氧(Reactive oxygen species,ROS)生成情况。结果:与对照组比较,姜黄素组各项指标无明显改变(P>0.05);与姜黄素组比较,高糖组Pink1、Parkin、LC3B、beclin1蛋白表达降低(P<0.01),细胞内ROS表达升高(P<0.01),线粒体膜电位活性下降(P<0.01);与高糖组比较,姜黄素高糖组Pink1、Parkin、LC3B、beclin1蛋白表达升高(P<0.01);细胞内ROS表达降低(P<0.01);线粒体膜电位活性升高(P<0.01)。结论:姜黄素可逆转高糖诱导的HK-2细胞线粒体自噬,抑制氧化应激。Objective:To observe the effects of curcumin on mitochondrial autophagy and oxidative stress in human renal tubular epithelial cells(HK-2)under high glucose environment,and to explore the protective effect and mechanism of curcumin on HK-2 cells.Methods:HK-2 cells were divided into 4 groups:control group(5.5 mmol/L glucose),curcumin group(5.5 mmol/L glucose+10μmol/L Cur),high glucose group(30 mmol/L glucose)and curcumin high glucose group(30 mmol/L glucose+10μmol/L Cur).All groups were stimulated for 48h.Total protein was collected,and the expression of mitochondrial autophagy related proteins Pink1,Parkin,LC3B and beclin1 was detected by Western blot.Mitochondrial membrane potential was detected by JC-1.Reactive oxygen species(ROS)detection kit was used to detect intracellular ROS production.Results:Compared with the control group,the indexes in the curcumin group did not change significantly(P>0.05).Compared with curcumin group,the protein expressions of Pink1,Parkin,LC3B and Beclin1 in high glucose group were significantly decreased(P<0.01),intracellular ROS expression increased significantly(P<0.01),and mitochondrial membrane potential activity decreased(P<0.01).Compared with high glucose group,the protein expression of Pink1,Parkin,LC3B and Beclin1 in curcumin high glucose group was significantly increased(P<0.01),intracellular ROS expression was significantly decreased(P<0.01),mitochondrial membrane potential activity increased(P<0.01).Conclusion:Curcumin can reverse the mitochondrial autophagy induced by high glucose and inhibit oxidative stress in HK-2 cells.

关 键 词：姜黄素 高糖 线粒体自噬 人肾小管上皮细胞 

分 类 号：R285.5[医药卫生—中药学]