Ori-Finder 2022:A Comprehensive Web Server for Prediction and Analysis of Bacterial Replication Origins  

作　　者：Mei-Jing Dong Hao Luo Feng Gao 

机构地区：[1]Department of Physics,School of Science,Tianjin University,Tianjin 300072,China [2]Frontiers Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering(Ministry of Education),Tianjin University,Tianjin 300072,China [3]SynBio Research Platform,Collaborative Innovation Center of Chemical Science and Engineering(Tianjin),Tianjin 300072,China

出　　处：《Genomics, Proteomics & Bioinformatics》2022年第6期1207-1213,共7页基因组蛋白质组与生物信息学报（英文版）

基　　金：supported by the National Key R&D Program of China(Grant No.2018YFA0903700);the National Natural Science Foundation of China(Grant Nos.21621004 and 31571358).

摘　　要：The replication of DNA is a complex biological process that is essential for life.Bacterial DNA replication is initiated at genomic loci referred to as replication origins(oriCs).Integrating the Z-curve method,DnaA box distribution,and comparative genomic analysis,we developed a web server to predict bacterial oriCs in 2008 called Ori-Finder,which is helpful to clarify the characteristics of bacterial oriCs.The oriCs of hundreds of sequenced bacterial genomes have been annotated in the genome reports using Ori-Finder and the predicted results have been deposited in DoriC,a manually curated database of oriCs.This has facilitated large-scale data mining of functional elements in oriCs and strand-biased analysis.Here,we describe Ori-Finder 2022 with updated prediction framework,interactive visualization module,new analysis module,and user-friendly interface.More species-specific indicator genes and functional elements of oriCs are integrated into the updated framework,which has also been redesigned to predict oriCs in draft genomes.The interactive visualization module displays more genomic information related to oriCs and their functional elements.The analysis module includes regulatory protein annotation,repeat sequence discovery,homologous oriC search,and strand-biased analyses.The redesigned interface provides additional customization options for oriC prediction.Ori-Finder 2022 is freely available at http://tubic.tju.edu.cn/Ori-Finder/and https://tubic.org/Ori-Finder/.

关 键 词：BACTERIA DNA replication Replication origin Z-curve DnaA-trio 

分 类 号：Q933[生物学—微生物学]