Purification and characterization of a cold-active myrosinase from marine Pseudomonas oleovorans SuMy07  

作　　者：Zhifa Huang Nannan Liu Yaowei Fang Xiaoyue Hou Guang Yang Jing Lu Haoyu Mi Qinwen Ye Rongjun Zhu Shu Liu 

机构地区：[1]Jiangsu Key Laboratory of Marine Bioresources and Environment,Jiangsu Ocean University,Lianyungang 222005,China [2]Co-Innovation Center of Jiangsu Marine Bio-industry Technology,Jiangsu Ocean University,Lianyungang 222005,China [3]School of Food Science and Engineering Jiangsu Ocean University,Lianyungang 222005,China [4]Jiangsu Marine Resources Development Research Institute,Lianyungang 222005,China

出　　处：《Acta Biochimica et Biophysica Sinica》2023年第4期695-699,共5页生物化学与生物物理学报（英文版）

基　　金：the grants from the National Natural Science Foundation of China(No.31772016);the Priority Academic Program Development of Jiangsu Higher Education Institutions,the Key Natural Science Foundation of the Jiangsu Higher Education Institutions of China(No.20KJA550001);Project″333″of Jiangsu Province and Jiangsu Natural Resources Development Special Project(Marine Science and Technology Innovation;No.JSZRHYKJ202008).

摘　　要：Myrosinase(EC 3.2.1.147),also known asβ-thioglucoside glucohydrolase,was first discovered in mustard seeds.Myrosinase hydrolyzes glucosinolate to produce D-glucose and an unstable aglycon,which upon rearrangement yields metabolites such as isothiocyanate,sulfate,acetonitrile,and thiocyanate.Isothiocyanate has a wide range of biological functions[1].Previous studies have shown that isothiocyanate has insect-repellant and antibacterial effects[2].Isothiocyanate also has anti-inflammatory and antioxidant properties,making it a potential anticancer compound[3,4].Sulforaphane is one of the most representative isothiocyanate substances against numerous cancers.Myrosinase extraction is technically challenging,although myrosinase metabolites have a variety of uses.At present,myrosinase is primarily extracted from white mustard seeds after a time-consuming and expensive preparation.Therefore,the search for myrosinase that can hydrolyze glucosinolates to produce isothiocyanate efficiently and economically has become an urgent task.Microbes can secrete myrosinase with rapid growth,lack of seasonal variation,simple intensive cultivation,and simple product extraction.

关 键 词：consuming REARRANGEMENT characterization 

分 类 号：O62[理学—有机化学]