复合EGFL7去细胞神经支架在周围神经损伤修复过程中的动物实验研究  

Animal experimental research of repairing sciatic nerve injury with compound EGFL7 decellularized neural scaffold

作　　者：殷超[1] 郝增涛[1] 王继宏[1] 王小龙[1] 霍志奇 YIN Chao;HAO Zengtao;WANG Jihong(Department of Hand and Foot Microsurgery Center,The Second Hospital Affiliated to Inner Mongolia Medical University,Huhhot,Inner Mongolia Autonomous,010090,China)

机构地区：[1]内蒙古医科大学第二附属医院手足显微外科中心,内蒙古自治区呼和浩特010090

出　　处：《实用手外科杂志》2023年第2期237-239,共3页Journal of Practical Hand Surgery

基　　金：内蒙古自治区自然科学基金项目(项目编号:2021LHMS08051);内蒙古医科大学青年创新基金项目(项目编号:YKD2020QNCX034)。

摘　　要：目的通过构建复合EGFL7植入物的去细胞神经支架移植修复大鼠坐骨神经损伤缺损模型,从而探究EGFL7在周围神经修复过程中促进血管化和神经恢复的作用。方法手术切取日本大耳白兔双侧胫神经,截成1.0 cm长的节段,制备去细胞神经支架。将制备好的外源性EGFL7的去细胞神经支架复合物植入大鼠坐骨神经损伤模型,于术后4周取标本行神经电生理检测、组织取材投射电镜形态学观察等来评价神经修复效果。结果添加外源性EGFL7模型组与不添加EGFL7的模型组相比再生的有髓神经纤维数量多、形态规则、直径粗、髓鞘厚、髓鞘不折叠或崩解、髓鞘发育良好;坐骨神经测得的诱发电位曲线波幅高、潜伏期短、神经传导速度快。结论EGFL7在周围神经修复过程中可起到促进血管化和神经功能恢复的作用。Objective To study the promotion of vascularization and neural recovery by EGFL7 in peripheral nerve repair,by constructing decellularized Neural Scaffolds witn complex EGFL7 which is used to repair rat sciatic nerve injury.Methods The dissection of the tibial nerves from the legs of Japanese white rabbits,which was to prepare decellularized neural scaffolds by cutting into 1.0 cm long segments.Implanted the prepared decellularized neural scaffold complex with exogenous EGFL7 into the rat sciatic nerve injury model,to Evaluate nerve repair effects by Nerve electrophysiological testing and projection electron microscope observation with specimens in 4 weeks after surgery.Results In the Model with the addition of exogenous EGFL7,the number of myelinated nerve fibers was large,the shape was regular,the diameter was thick,the myelin sheath was thick,the myelin sheath was not folded or disintegrated,and the myelin sheath was well developed,when compared with the model without the addition of exogenous EGFL7.Model with the addition of exogenous EGFL7 had the evoked potential curve measured by the sciatic nerve has high amplitude,short latency and fast nerve conduction velocity.Conclusion EGFL7 acts to promote vascularization and recovery of nerve function in the during peripheral nerve repair.

关 键 词：EGFL7 外周神经损伤 去细胞神经支架 血管化 

分 类 号：R651.3[医药卫生—外科学] R-332[医药卫生—临床医学]