草地早熟禾蛋白激酶CIPK24基因克隆及非生物胁迫响应分析  

Cloning of Protein Kinase CIPK24 Gene from Poa pratensis L.and Its Response to Abiotic Stresses

作　　者：陈阳[1,2] 尤学 刘文轩 高岩松 赵清峰 熊毅 金一锋[1,2] CHEN Yang;YOU Xue;LIU Wen-xuan;GAO Yan-song;ZHAO Qing-feng;XIONG Yi;JIN Yi-feng(College of Life Science and Agro-Forestry,Qiqihar Univesity,Qiqihar,Heilongjiang Province 161006,China;Heilongjiang Province Key Laboratory of Resistance Gene Engineering and Preservation of Biodiversity in Cold Areas,Qiqihar,Heilongjiang Province 161006,China)

机构地区：[1]齐齐哈尔大学生命科学与农林学院,黑龙江齐齐哈尔161006 [2]抗性基因工程与寒地生物多样性保护黑龙江省重点实验室,黑龙江齐齐哈尔161006

出　　处：《草地学报》2023年第6期1693-1701,共9页Acta Agrestia Sinica

基　　金：国家自然科学基金(31501785);黑龙江省自然科学基金项目(QC2017026);黑龙江省普通高等学校青年创新人才培养计划项目(UNPYSCT-2018102);齐齐哈尔大学教育科学研究项目(GJQTZX2021026)资助。

摘　　要：CIPK蛋白家族是Ca^(2+)介导的植物信号通路中关键蛋白家族,当植物受到逆境胁迫时,CBL-CIPK网络可调节离子运输进而适应逆境环境,其中CIPK24作用显著。为探究禾本科草坪草CIPK家族基因的作用,本研究以草地早熟禾(Poa pratensis L.)为试验材料,采用RT-PCR技术对草地早熟禾CIPK24基因进行克隆,并应用生物信息学及qRT-PCR技术,对非生物胁迫条件下该基因的表达方式及组织特异性进行研究。研究结果显示,草地早熟禾CIPK24包含典型的结构域CIPK_C,其属于AMPKA_Clike superfamily,与黑麦草(Lolium perenne L.),大麦(Hordeum vulgare L.),二粒小麦(Triticum turgidum L.)CIPK24同源性高达94.20%,90.62%,88.62%。草地早熟禾CIPK24基因的表达水平存在组织特异性,叶>茎>根。CIPK24基因积极响应干旱、氮素、磷素、盐胁迫,其中,无氮、低氮、无磷、高盐环境显著促进其表达。本研究为丰富草地早熟禾CIPK家族在非生物胁迫下的调节机制提供了理论基础。The CBL-interacting protein kinase(CIPK)family is a key protein family in the Ca^(2+)-mediated signaling pathway in plants.Under stresses,plants can adapt to the environmental stresses by regulating the ion transport by the CBL-CIPK network,of which CIPK24 takes a significant role.To explore the role of the CIPK gene family in Poaceae turfgrasses,Poa pratensis L.was used as the experimental material in this study.The CIPK24 gene of P.pratensis L.was cloned using RT-PCR,and its expression pattern and tissue specificity under abiotic stresses were investigated by bioinformatics and qRT-PCR.The results showed the CIPK24 from Poa pratensis L.contained a typical structural domain CIPK_C and belonged to the AMPKA_Clike superfamily,with the homology of 94.20%,90.62%and 88.62%of the CIPK24 from Lolium perenne L.,Hordeum vulgare L.and Triticum turgidum L.,respectively.The expression level of the CIPK24 gene in P.pratensis L.was tissue-specific:leaves>stems>roots.The CIPK24 gene in P.pratensis took active responses to the drought,nitrogen,phosphorus and salt stresses.Nitrogen-free,low-nitrogen,phosphorus-free and high-salt environments significantly promoted its expression.This study may provide a theoretical basis for the regulatory mechanism of the CIPK family in Poa pratensis L.under abiotic stresses.

关 键 词：草地早熟禾 蛋白激酶 CIPK24 非生物胁迫 表达分析 

分 类 号：Q344.13[生物学—遗传学]