Analysis of Key Physiological Characteristics of Portunus trituberculatus in Response to Short-Term Low Salinity Stress  

作　　者：ZHANG Xinni LIU Qi WANG Jiali GAO Gao NIU Mingming CHEN Lizhi WANG Huan WANG Fangfang WANG Chunlin MU Changkao 

机构地区：[1]School of Marine Science,Ningbo University,Ningbo 315211,China [2]Sanmen Agricultural and Rural Bureau,Taizhou 317100,China [3]Key Laboratory of Applied Marine Biotechnology,Ministry of Education,Ningbo University,Ningbo 315211,China

出　　处：《Journal of Ocean University of China》2023年第3期746-754,共9页中国海洋大学学报（英文版）

基　　金：supported by the National Key R&D Pro-gram of China(No.2020YFD0900203);the China Agricul-ture Research System of MOF and MARA;the K.C.Wong Magna Fund in Ningbo University。

摘　　要：Salinity is a significant environmental factor that can affect the survival,metamorphosis,growth and feeding of Portunus trituberculatus.In order to analyze the key physiological characteristics of P.trituberculatus in response to short-term low salinity stress,the experiments of gradually decline and recovery as well as abrupt decline in salinity were carried out.The results showed that P.trituberculatus could survive in a certain low salinity range in the short term,and salinity 12 was the lowest tolerable salinity under the present experimental conditions.The change of the hemolymph osmotic pressure displayed significant positive correlations with water salinity,and the pressure was always higher than seawater osmotic pressure.Short-term low salinity stress changed the structure and morphology of gill tissue.The expansion of gill filament ends and epithelial cell shedding were conducive to osmotic adjustment.The activities of key ion transport enzymes such as Na^(+)-K^(+)-ATPase,carbonic anhydrase and V-ATPase also changed with the osmotic regulation,while Na^(+)-K^(+)-ATPase played a dominant role.In summary,as an osmotic adjustment species,P.trituberculatus rapidly adapt to the short-term low-salinity environment by osmotic adjustment in vivo,but salinity below salinity 12 is not conducive to its survival.Our result enriched the theoretical mechanism of osmotic regulation of P.trituberculatus,providing reference for the development of aquaculture technology of P.trituberculatus.

关 键 词：Portunus trituberculatus gradual/abrupt decline in salinity GILL osmotic pressure ion transport enzymes 

分 类 号：S917.4[农业科学—水产科学]