Identification and Characterization of Long Non-Coding RNAs Involved in Sex-Related Gene Regulation in Kelp Saccharina japonica  

作　　者：BAI Meihan YUE Shutao WANG Weiwei DU Xinxin LI Xiaojie YANG Guanpin ZHANG Linan 

机构地区：[1]School of Marine Science and Engineering,Qingdao Agricultural University,Qingdao 266109,China [2]National Engineering Science Research&Development Center of Algae and Sea Cucumbers of China,Provincial Key Laboratory of Genetic Improvement&Efficient Culture of Marine Algae of Shandong,Shandong Oriental Ocean Sci-Tech Co.,Ltd.,Yantai 264003,China [3]Marine Science Research Institute of Shandong Province,Qingdao 266104,China [4]College of Marine Life Sciences,Ocean University of China,Qingdao 266003,China

出　　处：《Journal of Ocean University of China》2023年第3期755-765,共11页中国海洋大学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(No.132072962);the Agricultural Industrial Technology System in Shandong Province(No.SDAIT-26);the‘First Class Fishery Discipline’Programme[(2020)3]in Shandong Province;the Special Talent Programme‘Yishi Yiyi’in Shandong Province,China;the National Key R&D Program of China(No.2018YFD0901500);the Shandong Province Agriculture Seed Project(No.2021LZ GC004)。

摘　　要：Long non-coding RNAs(lncRNAs)regulate a variety of biological processes,including sexual reproduction and differentiation.Saccharina japonica,a commercially important brown alga in China,shows remarkable sexual dimorphism in haploid gametophytes.The sex of Saccharina japonica gametophytes is determined by UV sexual system.However,no results have been reported on the lncRNAs involved in the sex-related gene regulation of S.japonica.This study identified a number of lncRNAs and assessed their expression levels in male and female gametophytes.Among them,a total of 405 lncRNAs and 211 mRNAs showed differential expressions.Furthermore,the functions of target genes of differentially expressed lncRNAs(DELs)differed from those of differentially expressed genes(DEGs),suggesting that lncRNA may interact with other functional proteins,in addition to DEGs,to involve sex regulation in S.japonica.There were 32 and 90 potential cis-regulatory and trans-regulatory interactions between DELsDEGs,respectively.Five of these lncRNAs(LNC_002974,LNC_021059,LNC_038466,LNC_051584,and LNC_027400)interacted with putative male sex determination region(SDR)genes,suggesting that they act as regulators in gametophytes'sex regulation potentially.Findings from this study contribute to our understanding of the roles of lncRNAs in sex differentiation and lay the foundation for functional studies of candidate lncRNAs in the future.

关 键 词：Saccharina japonica long non-coding RNAs sex differentiation GAMETOPHYTE brown algae 

分 类 号：S917.3[农业科学—水产科学]