Identification and Pathogen Stimulation Patterns of Neuronal Nitric Oxide Synthase(nNOS)in Black Rockfish(Sebastes schlegelii)  

作　　者：YAN Xu WANG Ningning CAO Min LI Chao YANG Ning XUE Ting FU Qiang SONG Lin 

机构地区：[1]College of Chemistry and Molecular Engineering,Qingdao University of Science and Technology,Qingdao 266044,China [2]School of Marine Science and Engineering,Qingdao Agricultural University,Qingdao 266109,China [3]Shandong Provincial Key Laboratory of Biochemical Engineering,College of Marine Science and Biological Engineering,Qingdao University of Science and Technology,Qingdao 266042,China [4]Wuqiong Food Co.,Ltd.,Raoping 515726,China

出　　处：《Journal of Ocean University of China》2023年第3期842-850,共9页中国海洋大学学报（英文版）

基　　金：supported by the Natural Science Foundation of Shandong Province(No.ZR2020QC214);the Young Experts of Taishan Scholars(No.tsqn201909130);the Science and Technology Support Plan for Youth Innovation of Colleges and Universities in Shandong Province(No.2019KJF003);the‘First Class Fishery Discipline’Programme in Shandong Province;a special talent programme‘One Thing One Decision(YishiYiyi)’Programme in Shandong Province,China;the Breeding Plan of Shandong Provincial Qingchuang Research Team(2019)。

摘　　要：Neuronal nitric oxide synthase(nNOS)was the producer of nitric oxide(NO)which played important gas messenger molecules in biological process.It also can take effect as immune regulation molecule in organism.Black rockfish(Sebastes schlegelii)is an important economic fish which were widely farmed in East Asia countries.Meanwhile,the pathogenic bacteria such as the Edwardsiella tarda and Vibrio anguillarum in seawater always brought serious obstacles to their healthy growth.In order to explore the expression pattern of n NOS gene under the pathogen stimulation and predict its immune function,the n NOS gene in black rockfish named Ssn NOS was identified.It was 3780 bp in length,located on chromosome 6,and contained 27 coding domain sequence(CDs).According to the phylogenetic analysis,the Ssn NOS showed closest relative to the counterpart gene of swamp eel(Monopterus albus).Meanwhile,analysis of Ssn NOS expression in various healthy tissues showed that Ssn NOS expression level was highest in healthy brain tissues,followed by intestinal tissues.In addition,Ssn NOS showed significant expression changes in response to stimulation by two pathogens.Particular in gill,the expression of Ssn NOS after pathogenic stimulation increased significantly.The Elisa analysis showed the Ssn NOS content in gills was much higher than that in other tissues at all time points.Moreover,the expression patterns of Ssn NOS in brain,intestine and kidney after stimulation by pathogens showed a distinct expression pattern which first down-regulated and then up-regulated.Therefore,the Ssn NOS may be an important signaling molecule for fish to respond rapidly in immune stimulation.

关 键 词：neuronal nitric oxide synthase black rockfish immune function Edwardsiella tarda Vibrio anguillarum 

分 类 号：S943[农业科学—水产养殖]