基于代谢组学的瓦楞子及其伪品化学成分差异分析及细胞实验验证  

Metabolomics-based analysis of chemical composition differences between Arcae Concha and counterfeit product and cell experimental verification

作　　者：王巧珍 何晗 黄仕如 陈力源 刘友平[1] 陈鸿平[1] 陈林[1] WANG Qiao-zhen;HE Han;HUANG Shi-ru;CHEN Li-yuan;LIU You-ping;CHEN Hong-ping;CHEN Lin(State Key Laboratory of Southwestern Chinese Medicine Resources,Pharmacy College,School of Pharmacy,Chengdu University of Traditional Chinese Medicine,Chengdu 611130,China)

机构地区：[1]成都中医药大学药学院西南中药资源国家重点实验室,成都611130

出　　处：《天然产物研究与开发》2023年第6期925-937,共13页Natural Product Research and Development

基　　金：国家中医药管理局应用基础研究课题(gzyjc20210901)。

摘　　要：分析瓦楞子及其伪品的主要化学成分及其差异,验证差异化学成分药效作用。采用广泛靶向代谢组学技术检测瓦楞子及其伪品整体化学成分,通过聚类分析(HCA)、主成分分析(PCA)及正交偏最小二乘方判别分析(OPLS-DA)找出差异化学成分,结合细胞实验验证差异化学成分药效。共计检测出1208个代谢物,包括氨基酸及其代谢物、苯及其衍生物、杂环化合物等22类。其中正伪品共有成分1083个,正品中有而伪品中没有的成分40个。PCA结果显示不同基原正品之间可以被区分开,正品与伪品密肋粗饰蚶可以被区分开;差异代谢物分析发现,在正品中显著上调的差异代谢物有67个。细胞实验结果表明差异代谢物N^(6)-异戊烯基腺苷可促进人甲状腺癌细胞TPC-1的凋亡;通过诱导S、G2期阻滞抑制细胞增殖。N^(6)-异戊烯基腺苷可作为瓦楞子潜在的质量标志物。本研究为瓦楞子品质分析及质量控制提供技术支持。To analyze the main chemical composition and their differences of Arcae Concha and counterfeit product,and verify the efficacy of chemical composition.Widely targeted metabolomics technology was used to analyze the overall chemical composition of Arcae Concha and counterfeit product,identifying differential chemical compositions by cluster analysis(HCA),principal component analysis(PCA)and orthogonal partial least squares discriminant analysis(OPLS-DA).The efficacy of differential chemical compositions was verified by cell experiments.A total of 1208 compounds were identified from the samples,including 22 categories such as amino acids and their metabolites,benzene and its derivatives,and heterocyclic compounds.1083 common components were identified and 40 compounds in the genuine product but not in the fake product.The results of PCA showed that the different sources of the genuine products could be distinguished,the genuine products and counterfeit product could be distinguished;there were 67 differential metabolites that were significantly up-regulated in the genuine products.The results of cell test showed that N^(6)-Isopentenyladenosine can promote the apoptosis of human thyroid cancer cell line(TPC-1);through inducing S and G2 phase arrest to inhibit proliferationit.N^(6)-Isopentenyladenosine is expected to be a potential quality marker of Arcae Concha.This study provides technical support for quality analysis and control of Arcae Concha.

关 键 词：瓦楞子 混伪品 代谢组学 化学成分差异 人甲状腺癌细胞 

分 类 号：R284.1[医药卫生—中药学]