淮河安徽段秀丽白虾肠道菌群时空变化特征  

Temporal and spatial variations of intestinal microbiota of Exopalaemon modestus in Anhui section of Huai River

作　　者：张宁[1] 应聪萍 刘思磊 王银平[2] 罗俊义 刘凯[1,2,3] ZHANG Ning;YING Congping;LIU Silei;WANG Yinping;LUO Junyi;LIU Kai(Wuxi Fish-eries College,Nanjing Agricultural University,Wuxi 214081,Jiangsu,China;Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization,Ministry of Agriculture and Rural Affairs,Freshwater Fisheries Research Center,Chinese Academy of Fishery Sciences,Wuxi 214081,Jiangsu,China;National Demonstration Center for Experimental Station Fisheries Science Education,Shanghai Ocean University,Shanghai 201306,China)

机构地区：[1]南京农业大学无锡渔业学院,江苏无锡214081 [2]中国水产科学研究院淡水渔业研究中心,农业农村部淡水渔业和种质资源利用重点实验室,江苏无锡214081 [3]上海海洋大学,水产科学国家级实验教学示范中心,上海201306

出　　处：《生态学杂志》2023年第6期1426-1433,共8页Chinese Journal of Ecology

基　　金：省级财政项目“安徽省重点水域水生生物资源监测”(ZF2021⁃18⁃0786)资助。

摘　　要：为探究淮河干流安徽段底栖动物群落的肠道菌群时空分布特征,本研究利用16S rDNA扩增子测序技术,对区域优势虾类秀丽白虾的肠道菌群特征进行分析。结果表明:秀丽白虾肠道菌群共检出37门855属,其中假单胞菌属、短波单胞菌属、青枯菌属、柄杆菌属、乳球菌属和鞘脂单胞菌属为主要优势属;对比枯水期(3月)、平水期(6月)和丰水期(9月),秀丽白虾肠道菌群丰富度存在显著差异(P<0.05),β多样性指数显示从枯水期到丰水期各水域秀丽白虾肠道菌群的组成逐渐趋于相似;从枯水期到丰水期,各水域秀丽白虾肠道菌群共有ASVs逐渐增多,且在枯水期和平水期存在显著差异菌群(P<0.05),而丰水期则未发现;因不同水域生境差异及水文情势变动的影响,淮河干流安徽段秀丽白虾肠道菌群表现出规律的时空特性,各水域肠道菌群组成差异呈现为枯水期最大、平水期次之、丰水期则不显著的总体特征。To explore the temporal and spatial variations of the intestinal microbiota of benthic community in Anhui section of Huai River,16S rDNA amplicon sequencing technology was used to compare the intestinal microbiota characteristics of Exopalaemon modestus,one dominant shrimp species of the region.A total of 37 phylum and 855 genera of microbes were detected from E.modestus intestines,with Pseudomonas,Brevundimonas,Ralstonia,Cau⁃lobacter,Lactococcus and Sphingomonas as the dominant genera.There were significant differences in the intestinal microbiota richness of E.modestus(P<0.05)among low⁃water period(March),mean⁃flow period(June)and high⁃flow period(September).Theβdiversity index showed the intestinal microbiota composition in all sub⁃sec⁃tions tended to be similar from March to September.The common ASVs in intestines of E.modestus increased gradu⁃ally from low⁃water period to high⁃flow period.There were significant divergences of intestinal microbiota both in the low⁃water and mean⁃flow period(P<0.05),but not in the high⁃flow period.Due to the different habitats and hydro⁃logical regimes of different water bodies,the intestinal microbiota of E.modestus in Anhui section of Huai River(main stream)generally exhibited temporal and spatial variations.The intestinal microbiota composition of E.modestus in each sub⁃section showed the maximum divergence in low⁃water period,followed by mean⁃flow period,but no significant divergence in high⁃flow period.

关 键 词：秀丽白虾 水期 肠道菌群 差异性标志菌 

分 类 号：S917.4[农业科学—水产科学]