新疆兵团2021-2022年度B型Victoria系流感病毒基因组特征分析  

Genomic feature analysis of influenza B/Victoria in Xinjiang production and construction corps in 2021-2022

作　　者：郭景霞[1] 何尧 李红叶[1] GUO Jingxia;HE Yao;LI Hongye(Xinjiang Production and Construction Corps Center for Disease Control and Prevention,Urumqi,830002,China)

机构地区：[1]新疆生产建设兵团疾病预防控制中心检验科,新疆乌鲁木齐830002

出　　处：《中国病原生物学杂志》2023年第6期621-624,629,共5页Journal of Pathogen Biology

基　　金：2022年度兵团疾控中心研究项目(No.BTCDCKY202211)。

摘　　要：目的检测和分析新疆兵团2021-2022监测年度B/Victoria系流感病毒基因组特征及进化情况,为流感的防控提供科学依据。方法随机选取8株B/Victoria系荧光PCR检测核酸阳性标本,使用MDCK进行病毒的分离培养并提取RNA,通过全基因组深度测序获得B/Victoria系流感病毒8个基因序列,与世界卫生组织推荐的疫苗株等进行同源性分析;构建进化树,分析病毒进化情况。结果新疆兵团2021-2022监测年度B/Victoria系流感流行株之间血凝素HA基因的核苷酸同源性99.4%~99.9%,氨基酸同源性99.8%~99.9%。神经氨酸酶NA基因与疫苗株的核苷酸同源性99.6%~99.9%,氨基酸同源性99.8%~99.9%。其余6个内源基因的核苷酸及氨基酸同源性分别在99.0%~99.9%和99.7%~99.9%之间;HA、PB2、PB1、NP、MP基因进化分析显示,8株B/Victoria系流行株与疫苗株B/Austria/1359417/2021亲缘关系较近;NA、NS、PA基因进化树分析显示,8株B/Victoria系流行株与疫苗株B/Washington/02/2019亲缘关系较近。结论新疆兵团2021-2022监测年度B/Victoria系流感流行株属于V1A.3分支,与2022-2023年WHO推荐疫苗株B/Austria/1359417/2021亲缘关系较近,但仍然存在关键抗原表位漂变情况,而NA的氨基酸突变并未影响毒株的耐药性。Objective To identify and analyze the genome characteristics and evolution of the B/Victoria influenza virus in Xinjiang Corps during the 2021-2022 surveillance year,providing scientific basis for the prevention and control of influenza in the region.Methods 8 influenza B/Victoria strains which was detected positiveby Real-time reverse transcription polymerase chain reactionwere randomly selected,and MDCK cell was used to isolate and extract RNA from the positive samples.The gene sequences were obtained by whole-genome deep sequencing.Homology was tested withvaccine strains recommended by the World HealthOrganization.Phylogenetic trees was constructed toanalyze the evolution of the virus.Results The homology of thenucleotide and amino acid of hemagglutinin(HA)between B/Victoria influenza epidemic strains monitored in Xinjiang production and Construction Corps in 2021-2022 were 99.4%-99.9%and 99.8%-99.9%,respectively.And the homology of Neuraminidase(NA)were 99.6%-99.9%,and 99.8%-99.9%respectively.While the other six endogenous genes were 99.0%-99.9%/99.7%-99.9%respectively.The analysis of genetic evolution in HA,PB2,PB1,NP,and MP revealed that the eight B/Victoria strains were closely related to the vaccine strain B/Austria/1359417/2021,while the analysis of NA,NS,and PA showed that these strains were closely related to the vaccine strain B/Washington/02/2019.Conclusion The result suggest that the influenza epidemic strain of B/Victoria strain in Xinjiang production and Construction Corps in 2021-2022 belonged to the v1a.3 branch,which is closest to the vaccine strainB/Austria/1359417/2021(recommended by WHO in 2022-2023).There is still drift mutation in key antigen epitopes between the vaccine strains recommended by WHO,while the amino acid mutation of NA gene does not affect the drug resistance.

关 键 词：B/Victoria系流感病毒 血凝素 神经氨酸酶 基因特征 

分 类 号：R373.13[医药卫生—病原生物学]