六个外生菌根真菌菌株在不同难溶性磷源下的溶磷特性  

Phosphorus dissolving characteristics of six ectomycorrhizal fungal strains under different insoluble phosphorus sources

作　　者：江盈 邹锋[1] 黄建[2] 戴伟红 左荣花 田诗义 熊欢[1] JIANG Ying;ZOU Feng;HUANG Jian;DAI Weihong;ZUO Ronghua;TIAN Shiyi;XIONG Huan(Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees,Ministry of Education,Central South University of Forestry&Technology,Changsha 410004,Hunan,China;College of Forestry,Northwest A&F University,Yangling 712100,Shaanxi,China)

机构地区：[1]中南林业科技大学经济林培育与保护教育部重点实验室,湖南长沙410004 [2]西北农林科技大学林学院,陕西杨凌712100

出　　处：《菌物学报》2023年第6期1311-1329,共19页Mycosystema

基　　金：国家自然科学基金(32001309);湖南省教育厅优秀青年项目(21B0266);中南林业科技大学引进人才科研启动基金(2021YJ016)。

摘　　要：外生菌根真菌(ectomycorrhizal fungi,ECMF)可广泛与林木建立共生关系,促进林木对土壤磷的吸收。然而,不同菌种或菌株溶解不同磷源的能力不同,且影响菌株或菌种溶磷能力的生理因素尚不清楚。因此,本研究以从板栗Castanea mollissima和锥栗C.henryi林下采集的真菌子实体进行分离纯化及ITS序列的同源性分子鉴定所获得的5个菌株,和前期课题组获得的土生空团菌Cenococcum geophilum(CG)为研究对象,将其在以难溶性有机磷(植酸钙C_(6)H_(6)Ca_(6)O_(24)P_(6)、卵磷脂C_(42)H_(80)NO_(8)P)和难溶性无机磷(磷酸铝AlPO_(4)、磷酸铁FePO_(4))为磷源的蒙塔纳(Montana)培养基中进行培养,测定6个ECMF菌株在纯培养条件下的菌落直径、菌丝干质量、溶磷率、酸性磷酸酶活性、pH值、柠檬酸和草酸含量,分析在不同难溶性磷源下各菌株的生长及溶磷特性;并运用皮尔逊相关性分析,探讨不同难溶性磷源下影响菌株溶磷率的主要生理因素。结果表明:(1)6个ECMF菌株在不同难溶性磷下生长速率差异显著(P<0.05)。橙黄硬皮马勃Scleroderma citrinum(LY-20-2)在磷酸铝条件生长最快,中华豆马勃Pisolithus orientalis(LY-8)在其他3种难溶性磷源下生长均最快。(2)6个ECMF菌株对4种难溶性磷的溶磷能力具有显著差异(P<0.05)。以植酸钙为磷源时,LY-8溶磷率最高,达28.9%;以卵磷脂和磷酸铁为磷源时,LY-20-2的溶磷率最高,分别为3.2%和4.6%;以磷酸铝为磷源时,CG的溶磷率最高,达3.2%。(3)在4种难溶性磷源下,各菌株发酵液中的pH值均降低。以卵磷脂和磷酸铁为磷源时,除马勃Scleroderma sp.2(MB)以外,其他菌株均未分泌草酸。(4)以植酸钙为磷源时,菌落直径、菌丝干质量、酸性磷酸酶活性和草酸含量均与菌株溶磷率呈显著正相关(r=0.61–0.88);以卵磷脂为磷源时,菌落直径和酸性磷酸酶活性与菌株溶磷率呈显著正相关(r=0.50–0.95);以磷酸铝为磷源时,溶磷率与�Ectomycorrhizal fungi(ECMF)can widely form symbiosis with forest trees to promote the absorption of soil phosphorus.However,phosphate dissolving capacity of different fungal strains varied with different phosphorus sources,and the physiological factors affecting the dissolving capacity were still unclear.In this study,five fungal strains from Castanea mollissima and C.henryi forest were isolated,purified,and homology molecular identification was carried out on the basis of ITS sequencing.Together with Cenococcum geophilum(CG)obtained by the previous research,the strains were cultured on Montana medium containing insoluble organic phosphorus(calcium phytate C_(6)H_(6)Ca_(6)O_(24)P_(6),lecithin C_(42)H_(80)NO_(8)P and insoluble inorganic phosphorus(aluminum phosphate AlPO_(4),iron phosphate FePO_(4)).The colony diameter,dry mass of mycelium,phosphorus dissolving rate,acid phosphatase activity,pH value,citric acid and oxalic acid content of six ECMF strains were determined to analyze the growth and phosphorus dissolving characteristics of each strain under different insoluble phosphorus sources.Pearson correlation analysis was used to explore the main physiological factors affecting the phosphorus dissolving rate under different insoluble phosphorus sources.Results showed that the growth rates of six ECMF strains were significantly different on the medium with different insoluble phosphorus(P0.05).Scleroderma citrinum LY-20-2 grew fastest on the medium with AlPO_(4).On the media with other three insoluble phosphorus,Pisolithus orientalis LY-8 grew fastest.The capacities of six ECMF strains dissolving four kinds of insoluble phosphorus were significantly different(P0.05).When C_(6)H_(6)Ca_(6)O_(24)P_(6)was used as phosphorus source,LY-8 had the highest phosphorus dissolving rate of 28.9%.When C_(42)H_(80)NO_(8P)and FePO_(4)were used as phosphorus sources,the phosphorus dissolving rates of LY-20-2 were the highest,reaching 3.2%on the medium with C_(42)H_(80)NO_(8)P and 4.6%on the medium with FePO_(4).When AlPO_(4)was

关 键 词：外生菌根真菌 分离鉴定 酸性磷酸酶 有机酸 解磷 

分 类 号：S714.3[农业科学—林学]